Sensor for Alpha Pinene by Using Quartz Crystal Microbalance Coated with Molecular Imprinted Polymer

SISILIA AYU KEZIA H, Drs. Iqmal Tahir, M.Si; Dr. Eng. Kuwat Triyana, M.Si

2019 | Skripsi | S1 KIMIA

Pembuatan sensor alfa pinene dilakukan dengan menggunakan Quartz Crystal Microbalance (QCM) yang dilapisi dengan Molecular Imprinted Polymer (MIP). Penelitian ini dilakukan untuk mengetahui efektivitas sensor QCM yang telah dilapisi dengan MIP dalam analisis alfa pinene dan dengan cepat memberikan metode alternatif untuk analisis alfa pinene. Penelitian dimulai dengan preparasi MIP. MIP dibuat dengan mereaksikan alfa pinene dengan asetonitril, EGDMA, dan AIBN. Setelah MIP terbentuk, MIP diekstrak untuk menghapus template. Selanjutnya, larutan MIP disiapkan dengan mencampur PAN, DMF, dan MIP. Setelah larutan MIP siap, sensor QCM disiapkan. QCM sensor dicuci dengan larutan piranha dan air suling secara berurutan. Kemudian, MIP dilapisi menggunakan teknik spin coating. Setelah itu, MIP dilapisi QCM dikarakterisasi dengan menggunakan AFM. Kemudian, respon sensor QCM dievaluasi dengan menggunakan larutan standar alfa pinene. Setelah kurva kalibrasi perubahan frekuensi resonansi vs volume diperoleh, selektivitas dari QCM yang telah terlapis oleh MIP diamati dengan menggunakan alfa-pinene, beta-pinene, dan gama-terpinene. Selanjutnya, analisis kualitatif dilakukan dengan menggunakan minyak eucalyptus. QCM yang telah dilapisi oleh MIP dapat digunakan sebagai metode alternatif untuk analisis kualitatif alfa-pinene. Persamaan garis lurus dari QCM yang dilapisi oleh MIP adalah y = 24,1 x + 24,7 dan R^2 = 0,9788. Sensitivitas dari metode ini adalah 24,1 Hz/uL. LoD dan LoQ metode ini adalah 0,066 dan 0,219 uL. Waktu respon dari alfa-pinene, beta-pinene, dan gama-terpinene adalah 150, 108, dan 97 detik, dan humiditas tidak mempengaruhi hasil analisis pada sensor. QCM yang telah dilapisi oleh MIP menunjukkan hasil yang selektif untuk analisis alfa-pinene.

Preparation of alpha-pinene sensor based on Quartz Crystal Microbalance (QCM) coated with Molecular Imprinted Polymer (MIP) was done. This research was done to know the effectiveness of QCM sensor that has been coated with MIP and rapidly provide an alternative method for analysis of alpha-pinene. The research began with the synthesis of MIP. The MIP was synthesized by reacting alpha-pinene with acetonitrile, ethylene glycol dimethacrylate, and 2,2-azobis-2-methylpropyonitrile. After the MIP formed, it was extracted to remove the template. Next, the MIP solution was prepared by mixing polyacrylonitrile, dimethylformamide, and MIP. After the MIP solution ready, the QCM sensor was prepared. The QCM sensor was washed with piranha solution and distilled water respectively. Then, the MIP was used to coat QCM surface by spin coating technique. The QCM coated MIP was characterized by using AFM. Then, the QCM respond sensor was evaluated by using alpha-pinene standard solution. After the calibration curve of resonance frequency shift vs volume was obtained, the selectivity of QCM based MIP was observed by using alpha-pinene, beta-pinene, and gamma-terpinene. Next, the test of qualitative analysis was done by using sample. The QCM that had been coated by MIP could be used as an alternative method for qualitative analysis of alpha-pinene.The straight line equation of QCM coated by MIP was y=24.1x + 24.7 and R^2= 0,9788. The sensitivity of this method was 24.1 Hz/uL. The LoD and LoQ of this method were 0.066 and 0.219 uL. The respond times of the alpha-pinene, beta-pinene, and gamma-terpinene were different, they were 150, 108, and 97 seconds, and the humidity did not affect the analysis of the sensor. The QCM that had been coated by MIP showed that it was selective to alpha-pinene.

Kata Kunci : alpha-pinene; chemical sensor; molecular imprinted polymer; quartz crystal microbalance