PENGARUH KONSENTRASI EKSTRAK DAUN KEMANGI (Ocimum basilicum L.) TERHADAP PERTUMBUHAN Staphylococcus aureus PADA PLAT GIGI TIRUAN THERMOPLASTIC NYLON

MARIA RISTAULI S, Dr. drg. Endang Wahyuningtyas, M.S., Sp. Pros(K); drg. Maria Theresia Esti Tjahjanti, M.Kes., Sp. Pros(K)

2019 | Skripsi | S1 KEDOKTERAN GIGI

Thermoplastic nylon salah satu bahan plat gigi tiruan yang memiliki kekurangan yaitu bersifat mudah menyerap air sehingga menyebabkan permukaan plat gigi tiruan menjadi kasar, memungkinkan mudah terjadi pertumbuhan bakteri Staphylococcus aureus. Daun kemangi (Ocimum basilicum L.) mengandung senyawa aktif sebagai antibakteri seperti tanin dan flavonoid. Tujuan dari penelitian ini adalah untuk mengkaji pengaruh konsentrasi ekstrak daun kemangi terhadap pertumbuhan Staphylococcus aureus pada plat gigi tiruan thermoplastic nylon. Penelitian ini menggunakan spesimen thermoplastic nylon sebanyak 24 buah berupa cakram dengan diameter 10 mm dan tebal 1,5 mm. Subjek penelitian direndam dalam saliva buatan selama 1 jam, setelah itu direndam dalam suspensi Staphylococcus aureus dalam media Brain-Heart Infusion Broth (BHI-B) selama 24 jam pada suhu 37���°C. Subjek penelitian dibagi menjadi empat kelompok: kelompok kontrol dengan akuades steril, konsentrasi ekstrak daun kemangi 6,25%, 12,5%, dan 25%. Masing-masing kelompok direndam selama 15 menit. Sebanyak 0,01 ml dari larutan pengenceran 10-3 ditanam dalam media Mannitol Salt Agar (MSA) dan diinkubasi selama 24 jam pada suhu 37���°C. Subjek penelitian dihitung jumlah koloni Staphylococcus aureus menggunakan colony counter. Data yang diperoleh dilakukan analisa menggunakan One-way Anova dan uji LSD. Hasil analisis One-way Anova menunjukkan terdapat perbedaan bermakna konsentrasi ekstrak daun kemangi 6,25%, 12,5%, dan 25% terhadap pertumbuhan Staphylococcus aureus (p <0,05). Hasil analisis LSD menunjukkan perbedaan bermakna antar kelompok perlakuan pada plat gigi tiruan thermoplastic nylon (p <0,05). Kesimpulan dari penelitian adalah konsentrasi ekstrak daun kemangi 25% paling berpengaruh dalam menghambat pertumbuhan Staphylococcus aureus pada plat gigi tiruan thermoplastic nylon.

Thermoplastic nylon one of denture base material that has a deficiency which is to easily absorb water which cause the surface of the denture to become rough, allows the bacteria Staphylococcus aureus to grow easily. Basil leave (Ocimum basilicum L.) contain active compound as an antibacterial such as tannin and flavonoid. The purpose of this research is to examine the effect of concentration of basil leaf extract on the growth Staphylococcus aureus of thermoplastic nylon denture base. This research used 24 pieces of thermoplastic nylon in the form of disc with a diameter of 10 mm and 1.5 mm of thickness. The research subject is soaked in artificial saliva for 1 hour, and then it is soaked in Staphylococcus aureus in a media of Brain-Heart Infusion Broth (BHI-B) for 24 hours at 37���°C. The research subject is divided into four groups: control group with sterile aquades, concentration of basil leaf 6.25%, 12.5%, and 25%. Each group soaked for 15 minutes. As much as 0.01 ml of 10-3 dilution solution was planted in Mannitol Salt Agar (MSA) media and incubated for 24 hours at 37���°C. The research subject was calculated in the numbers of Staphylococcus aureus colonies using colony counter. The data obtained is analyzed using One-way Anova and LSD test. The result of One-way Anova analysis showed that there are significant differences in the effect of the basil leaf extract concentration of 6.25%, 12.5% and 25% on the growth of Staphylococcus aureus (p >0.05). LSD analysis result showed significant differences between treatment groups on thermoplastic nylon denture base (p >0.05). The conclusion of the research is that the basil leaf concentration of 25% has the most influence in inhibiting the growth of Staphylococcus aureus on thermoplastic nylon denture base.

Kata Kunci : thermoplastic nylon, Staphylococcus aureus, daun kemangi (Ocimum basilicum L.)