IN-VITRO STUDY OF NON-STEROIDAL ANTI INFLAMATORIC DRUGS ON THE EXPRESSION OF COX-2 MRNA IN COLONIC FIBROBLAST OF RAT

JONATHAN, dr. Dwi Aris Agung, N M.Sc, PhD ; dr. Eko Purnomo, PhD; drg. Fara Silvia Yuiani, M.Sc

2019 | Skripsi | S1 KEDOKTERAN

Background : Non-Steroidal Anti-Inflammatory Drugs (NSAIDs) are often given to post-operative patients to reduce pain and discomfort. Pain and discomfort are mostly mediated by prostaglandins E2 which is produced by cyclooxygenase-2 enzyme through conversion of arachidonic acid. However, NSAIDs are also known to relate with Anastomosis leakage. Blockage of Cyclooxygenase-2 (COX-2) by NSAIDs to prevent inflammatory reaction reduces prostaglandins E2 conversion which plays major role in wound healing, and block the further cascade of wound healing. Objective : The aim of the study is to investigate the effect of NSAIDs on expression of COX-2 mRNA on colonic fibroblast of rat compared with control. Method : This study used colonic fibroblast culture isolated from Sprague Dawley rat as subject, which divided into paracetamol treated group, metamizole treated group, ketorolac treated group, and negative control. Then the mRNA of COX-2 expression was assessed by PCR and electrophoresis. Results : The COX-2 mRNA was expressed on ketorolac with dosage of 25μg/mL, Metamizole with dosage of 5μg/mL and 50μg/mL. Meanwhile, COX-2 mRNA expression is not seen on the control groups, metamizole 50μg/mL group and paracetamol treated groups. Conclusion : There was difference of COX-2 mRNA expression. It is not expressed on colonic fibroblast culture treated with paracetamol, low dosage of ketorolac and high dose of metamizole

Background : Non-Steroidal Anti-Inflammatory Drugs (NSAIDs) are often given to post-operative patients to reduce pain and discomfort. Pain and discomfort are mostly mediated by prostaglandins E2 which is produced by cyclooxygenase-2 enzyme through conversion of arachidonic acid. However, NSAIDs are also known to relate with Anastomosis leakage. Blockage of Cyclooxygenase-2 (COX-2) by NSAIDs to prevent inflammatory reaction reduces prostaglandins E2 conversion which plays major role in wound healing, and block the further cascade of wound healing. Objective : The aim of the study is to investigate the effect of NSAIDs on expression of COX-2 mRNA on colonic fibroblast of rat compared with control. Method : This study used colonic fibroblast culture isolated from Sprague Dawley rat as subject, which divided into paracetamol treated group, metamizole treated group, ketorolac treated group, and negative control. Then the mRNA of COX-2 expression was assessed by PCR and electrophoresis. Results : The COX-2 mRNA was expressed on ketorolac with dosage of 25μg/mL, Metamizole with dosage of 5μg/mL and 50μg/mL. Meanwhile, COX-2 mRNA expression is not seen on the control groups, metamizole 50μg/mL group and paracetamol treated groups. Conclusion : There was difference of COX-2 mRNA expression. It is not expressed on colonic fibroblast culture treated with paracetamol, low dosage of ketorolac and high dose of metamizole

Kata Kunci : NSAIDs, COX-2 mRNA, Anastomosis leakage, post-test only study group design, in-vitro study, colonic fibroblast of rat