Uji Aktivitas Fraksi Etil Asetat Ekstrak Etanolik Herba sambiloto (Andrographis paniculata (Burm.f) Ness) Terhadap Kualitas Sperma Mencit Balb/c In Vivo

NOVENDRA ALIM W, Prof. Dr. Suwijiyo Pramono, DEA., Apt.; Prof. Dr. Agung Endro Nugroho, M.Si., Apt.

2018 | Skripsi | S1 FARMASI

Berbagai penelitian terdahulu menyebutkan ekstrak etanolik herba sambiloto terbukti memiliki efek farmakologi yang luas. Selain memiliki efek antiinflamasi dan antidiabetes juga terbukti dapat mempengaruhi spermatogenesis. Efek yang ditimbulkan berdampak pada menurunnya jumlah dan motilitas sperma serta peningkatan abnormalitas sperma. Pada penelitian kali ini bertujuan untuk mengetahui apakah fraksi etil asetat ekstrak etanolik herba sambiloto memiliki pengaruh dalam menurunkan jumlah dan motilitas sperma serta peningkatan abnormalitas sperma mencit Balb/c. Penelitian ini menggunakan mencit jantan galur Balb/c sebanyak 25 ekor berusia 30 hari yang dibagi kedalam 5 kelompok uji. Kelompok ekstrak sambiloto (dosis 1500 mg/kg BB), fraksi etil asetat ekstrak sambiloto (dosis 50,949 mg/kg BB dan dosis 25,641 mg/kg BB), kontrol positif menggunakan ekstrak gandarusa (dosis 0,195 mg/kg BB), dan kontrol negatif dengan CMC-Na 0.5%. Pemberian dilakukan secara oral selama 35 hari. Pada hari ke 36 dilakukan pembedahan dan pemeriksaan farmakologi berupa jumlah/konsentrasi sperma, abnormalitas sperma, dan motilitas sperma mencit Balb/c. Hasil penelitian fraksi etil asetat dengan dosis 50,949 mg/kg BB memberikan pengaruh paling baik terhadap penurunan motilitas dan kenaikan abnormalitas sperma mencit. Fraksi etil asetat dengan dosis 25,641 mg/kg BB dan dosis 50,949 mg/kg BB tidak memberikan pengaruh terhadap jumlah/konsentrasi sperma mencit jantan galur Balb/c

Many former investigations mentioning that the ethanolic extract of Sambiloto herb is proven in having a broad pharmacology effects. Beside of having anti-inflammation and anti-diabetes effects, it also affects spermatogenesis. The effect results in decreasing the amount and motility of the sperm and increasing of the sperm abnormality. This examination aims to investigate the effect of the ethyl acetate fraction of Sambiloto herb ethanolic extract in decreasing the amount and motility of the sperm and increasing of the sperm abnormality of Balb/c mice. The examination used 25 male Balb/c mice aging 30 days which were divided into 5 examination groups. They were: the Sambiloto extracts group (dosage 1500 mg/kg BB); the ethyl acetate fraction of Sambiloto extract (dosage 50.949 mg/kg BB) group; the ethyl acetate fraction of Sambiloto extract (dosage 25.641 mg/kg BB) group; the positive control group employing Gandarusa extract (dosage 0.195 mg/kg BB); and the negative control group employing CMC-Na 0.5%. The injections were given in 35 days. In the 36th day, surgery and pharmacology examination were done in order to investigate the amount/concentration, abnormality, and the motility of the Balb/c mice sperm. The result showed that the ethyl acetate fraction with the dosage of 50.549 mg/kg BB gave the best impact towards the motility reduction, and the abnormality improvement of the sperm of mice. Meanwhile, the ethyl acetate fractions with the dosage of 25.641 and 50.949 mg/kg BB give no effect towards the amount/concentration of the sperm of the male Balb/c mice.

Kata Kunci : Herba sambiloto, andrografolid, fraksi etil asetat, kualitas sperma