KADAR KREATININ, UREUM, DAN ASAM URAT DALAM DARAH TIKUS PUTIH (Rattus norvegicus, Berkenhout, 1769) BETINA DENGAN PERLAKUAN Arthrosipra maxima Setchel et Gardner DAN Chlorella vulgaris Beijerinck

ROHMI SALAMAH, Dra. Mulyati Sarto, M.Si

2018 | Skripsi | S1 BIOLOGI

Arthrospira maxima dan Chlorella vulgaris digunakan sebagai suplemen karena kandungan proteinnya yang sangat tinggi. Protein merupakan makromolekul yang memiliki gugus nitrogen. Metabolisme nitrogen menghasilkan produk akhir diantaranya kreatinin, ureum, dan asam urat. Produk akhir tersebut harus diekskresikan dari tubuh melalui ginjal. Sehingga, kadar zat tersebut dapat dijadikan sebagai indikator fungsi ginjal. Penelitian ini bertujuan untuk mengetahui pengaruh pemberian A. maxima dan C. vulgaris terhadap fungsi ginjal setelah perlakuan selama 28 hari. Penelitian ini menggunakan hewan model Rattus norvegicus sebanyak 15 ekor. Hewan model dibagi menjadi 3 kelompok, yaitu kelompok kontrol, kelompok A. maxima dan kelompok C. vulgaris. Dosis A. maxima dan C. vulgaris sama yaitu 5000mg/kgBB. Pengamatan dilakukan setiap 7 hari dengan parameter kreatinin, ureum, dan asam urat dalam darah. Analisis data menggunakan uji statistik one way ANOVA dan uji lanjutan DMRT. Hasil penelitian menunjukan A. maxima menurunkan kadar kreatinin (32%), ureum (11%) dan asam urat (3%). C. vulgaris menurunkan kadar kreatinin (19%), serta meningkatkan kadar ureum (11%) dan asam urat (35%).

Arthrospira maxima and Chlorella vulgaris are used as supplement as their high protein content. Protein is macromolecule that has nitrogen group. Nitrogen metabolism produces end products that must be excreted, they are creatinine, ureum, and uric acid. Thus end products are excreted through kidney, so the level in the blood can be kidney function indicator. The aim of this study was to know the effect of A. maxima and C. vulgaris on kidney function after 28 days treatment. Fifteen female wistar rats (Rattus norvegicus) were grouped into 3 groups which were control and treatments of A. maxima, and C. vulgaris. Each group respectively was administered by oral gavage with 0 (control) or 5000 mg/kg.bw/day of A. maxima and C. vulgaris suspended in aquadest. Observation was carried out every 7 day with creatinine, ureum, and uric acid of rat blood as parameters. One way ANOVA followed by DMRT test were used to analyze the data. A. maxima decreased level of creatinine (32%), ureum ( 11%), and uric acid (3%). C. vulgaris decreased creatinine level (32%), as well as increased level of ureum (11%) and uric acid (35%).

Kata Kunci : Arthrospira maxima, Chlorella vulgaris, Creatinine, Ureum, Uric acid