PENGARUH KEJU SINBIOTIK TERHADAP EKSPRESI GEN DAN KADAR PROTEIN Intercellular Cell Adhesion Molecule-1 (ICAM-1) LAMBUNG TIKUS YANG TERPAPAR INDOMETASIN

SALMI, Dr. Sunarti, M.Kes.; Prof. Dr. Nurliyani, M.S.

2018 | Tesis | MAGISTER ILMU BIOMEDIK

Latar belakang : Peningkatan ekspresi Intercellular Cell Adhesion Molecule-1 (ICAM-1) terlibat dalam patogenesis kerusakan lambung akibat penggunaan indometasin. Penekanan ekspresi ICAM-1 dapat mencegah kerusakan lambung akibat penggunaan indometasin. Keju sinbiotik merupakan sumber peptida bioaktif yang diperkaya dengan bakteri starter Lactobacillus rhamnosus dan penambahan glukomanan porang berpotensi sebagai pangan fungsional yang memiliki aktivitas anti-inflamasi Tujuan : Penelitian ini bertujuan untuk mengetahui pengaruh keju sinbiotik terhadap ekspresi gen dan kadar protein ICAM-1 lambung tikus yang terpapar indometasin. Metode: Penelitian menggunakan tikus jantan galur Wistar yang dikelompokkan kedalam 6 kelompok perlakuan: normal (N), Indometasin (IND): Keju Probiotik (KP), Keju sinbiotik 0,36g/hari (KS I), Keju sinbiotik 0,72 g/hari (KS II) dan Keju sinbiotik 1,44 g/hari (KS III). Pemberian keju sinbiotik atau keju probiotik pada kelompok KS dan KP dilakukan selama 28 hari sedangkan kelompok N dan IND diberi air desilat. Lambung tikus diberi paparan indometasin dengan dosis 20 mg/kg BB pada hari ke 29 dan dikorbankan 24 jam berikutnya. Tingkat ekspresi gen dan kadar protein ICAM-1 lambung ditentukan menggunakan real-time PCR dan teknik ELISA. Hasil : Paparan indometasin menyebabkan peningkatan ekspresi gen dan protein ICAM-1 yang signifikan (p<0,05) pada kelompok indometasin. Pemberian keju sinbiotik selama 28 hari sebelum paparan indometasin dapat mencegah peningkatan ekspresi gen dan kadar protein ICAM-1 lambung tikus. Ekspresi gen ICAM-1 dapat ditekan secara signifkan (p<0,05) oleh keju sinbiotik dengan dosis 0,36g/hari dan 0,72 g/hari, sedangkan penekanan kadar protein ICAM-1 yang signifikan (p<0,05) hanya terdapat pada kelompok yang menerima keju sinbiotik dengan dosis 0,72 g/hari. Kesimpulan : Keju sinbiotik dengan dosis 0,72 g/hari mampu menekan peningkatan ekspresi gen dan kadar protein ICAM-1 lambung tikus yang terpapar indometasin.

Background : Up-regulation of Intercellular Cell Adhesion Molecule -1 (ICAM1) was involved in pathogenesis of indomethacin-induced gastric injury. Downregulation ICAM-1 could be an alternative to prevent indomethacin-induced gastric injury. Synbiotic cheese is a source of milk-bioactive peptide and was enriched with bacteria starter Lactobacillus rhamnosus and additional of porang glucomannan which potent as fuctional food with anti-inflammatory properties. Objectives : This study aimed to determine the effect of synbiotic cheese on gene expression and protein levels of ICAM-1 on exposured of indomethacin in rat gastric Methods : The study used male Wistar rats grouped into 6 treatment groups: normal (N), Indomethacin (IND), Probiotic cheese (KP), Synbiotic cheese 0,36 g/day (KS I), synbiotic cheese 0,72 g/day (KS II) and Synbiotic cheese 1,44 g/day (KS III). KS dan KP group was pre-treatment with synbiotic cheese or probiotic cheese for 28 days, while N and IND group received distillate water only. Rats gastric exposed to indomethacin on 29 h day using 20 mg/kg bw indomethacin. Rats were euthanized 24 hours after indomethacin treatment and then the gastric tissue was took. Relative ICAM-1 gene epressions were quantified using realtime PCR, while the protein levels of ICAM-1 were quantified using ELISA techique. Result : Indomethacin induced significantly (p<0,05) both in gene expression and protein levels of ICAM-1 in gastric tissue. Pre-treatmen with synbiotic cheese for 28 days showed suppression effect on ICAM-1 expression that significantly different to indomethacin group. Synbiotic cheese at dose 0,36 g/day showed significant suppression effect on ICAM-1 gene expression, but no significant suppression in protein levels of ICAM-1. Synbiotic cheese at dose 0,72 g/day showed significant suppression effect on ICAM-1 expression, both in gene expression and protein levels of ICAM-1. Both gen expression and protein levels of ICAM-1 in synbiotic cheese group was lower than probiotic cheese grups, but it was not statistically different (p>0,05). Conclusion: Synbiotic cheese at dose 0,72 g/day dose suppressed up-regulation both gene expression dan protein levels of ICAM-1 on indomethacin exposured in rat gastric

Kata Kunci : Ekspresi gen ICAM-1, kadar protein ICAM-1, keju sinbiotik, indometasin, ICAM-1 gene expression, protein levels of ICAM-1, Synbiotic cheese, Indomethacin