Uji Antikonstipasi Sampel Larutan Bahan Alam pada Tikus Putih Wistar Terinduksi Loperamid

LENY DWI LESTARI, Dr. Purwantiningsih, M.Si., Apt.

2018 | Skripsi | S1 FARMASI

Sampel larutan bahan alam diyakini dapat memberikan efek terapi sebagai antikonstipasi. Efek ini belum diketahui secara pasti, karena uji antikontipasi terhadap sampel larutan bahan alam ini belum pernah dilakukan sebelumnya. Sehingga, penelitian dilakukan untuk mengetahui efek antikonstipasi dari sampel larutan bahan alam tersebut. Hewan uji yang digunakan adalah tikus putih jantan dewasa galur Wistar. Uji dimulai dengan adaptasi hewan uji selama 3 hari dan pembagian kelompok menjadi kelompok kontrol normal, kontrol negatif, kontrol positif dan 3 kelompok uji. Semua kelompok uji kecuali kelompok kontrol normal diinduksi konstipasi dengan memberikan suspensi loperamid 3 mg/kgBB selama 3 hari. Selama 7 hari berikutnya, kelompok hewan uji diberikan larutan uji, kelompok kontrol positif diberikan suspensi bisakodil dengan dosis 0,25 mg/kgBB, kelompok kontrol normal dan kontrol negatif diberikan aquadest sebanyak 1 mL per ekor hewan uji. Selama 7 hari tersebut, dilakukan pengukuran terhadap jumlah feses, bobot feses, dan kadar air feses. Pada hari terakhir pemberian larutan uji, dilakukan pengukuran gastrointestinal transit rasio (GIT rasio) dengan bantuan suspensi fenol red dalam CMC Na. Hasil uji antikonstipasi terhadap sampel larutan bahan alam menunjukkan bahwa larutan uji ini tidak dapat meningkatkan jumlah, bobot, dan kadar air feses, serta gastrointestinal transit rasio pada tikus putih wistar yang mengalami konstipasi. Sehingga berdasarkan hasil ini, dapat disimpulkan bahwa larutan uji tidak dapat memberikan efek antikonstipasi pada tikus putih wistar yang mengalami konstipasi.

The natural materials solution sample is believed to provide therapeutic effects as anti constipation. This effect is not known for certain, because the natural materials solution sample has not been tested before. Thus, the study was conducted to investigate the anti constipation effect of the natural materials solution sample. The animal used for test is the adult white male wistar rat. The test was started with 3 days of animal adaptation and division of group into normal control group, negative control group, positive control group and 3 test groups. All the test groups except the normal control group were induced constipation by giving a loperamide suspension 3 mg/kg of body weight for 3 days. For the next 7 days, the test animal group was given the test solution, positive control group was given bisacodyl suspension 0.25 mg/kg of body weight, normal control group and negative control group were administered 1 mL of aquadest for every animal test. During the 7 days, measurements were made on the number of feces, feces weight, and fecal water content. On the last day of the test, gastrointestinal transit was measured with the phenol red suspension in CMC Na as a marker. Anti constipation test results against the natural materials solution sample showed that this solution could not increase the amount, weight, and moisture content of the feces, as well as the gastrointestinal transit ratio in constipated rats. Thus, based on these results, it can be concluded that the test solution can not provide anti constipation effect in constipated rats.

Kata Kunci : larutan bahan alam, antikonstipasi, loperamid, natural materials solution, anti constipation, loperamide