Efek Pemberian Hemolymph Kepiting Bakau (Scylla serrata) pada Infeksi Methicillin Resistant Staphylococcus aureus secara In Vitro dan In Vivo

ADITYA HARINTO P, Prof. Dr. drh. Siti Isrina Oktavia Salasia

2018 | Skripsi | S1 KEDOKTERAN HEWAN

Kasus infeksi Methicillin Resistant Staphylococcus aureus (MRSA) yang telah resisten terhadap sebagian besar antibiotika beta-lactame menyebabkan sepsis dan toxic shock syndrome yang fatal. Scylla serrata atau kepiting bakau dikenal mampu hidup dalam lingkungan air tercemar penuh dengan mikroorganisme patogen, karena memiliki imunitas non spesifik berupa Antimicrobial Peptides (AMPs) dalam sirkulasi hemolymph/darah. Penelitian bertujuan mengevaluasi aktivitas antibakteri hemolymph kepiting bakau terhadap infeksi sistemik MRSA secara in vitro dan in vivo. Uji in vitro, pemberian hemolymph konsentrasi bertingkat 1%-50% dengan doxycycline sebagai kontrol pada paper disc, didapatkan konsentrasi terendah hemolymph dengan aktivitas antibakteri optimal adalah 10% berukuran DDH sebesar 12±0,62 mm. Konsentrasi hemolymph optimal (10%) dipilih untuk dibuat sediaan basis dextrosa. Uji in vivo dilakukan dengan menggunakan 15 ekor mencit yang diinfeksikan dengan MRSA 800 mikroliter 10 pangkat 8 CFU secara intraperitoneal dan 3 hari kemudian dilanjutkan dengan 40 mikroliter 10 pangkat 8 CFU MRSA intravena. Mencit dibagi dalam 3 kelompok, kelompok I (kontrol placebo), kelompok II (doxycycline), dan kelompok III (perlakuan hemolymph) diberi setiap hari selama 15 hari. Pemberian sediaan hemolymph 10% selama 15 hari mampu meredakan gejala infeksi sistemik MRSA yang didukung dari hasil histopatologi organ. Sediaan Hemolymph 10% memiliki aktivitas antibakteri terhadap infeksi sistemik MRSA secara in vitro dan in vivo

The case of systemic infection of Methicillin Resistant Staphylococcus aureus (MRSA) that has been resistant to most beta-lactame antibiotic cause fatal sepsis and toxic syndrome. Scylla serrata or mud crabs have complex immune system to survive in a micro-pathogenic tainted water environment, because they have a non-specific immunity of Antimicrobial Peptides (AMPs) in the hemolymph/blood circulation. The study aims to evaluate the crab blood antibacterial activity against MRSA systemic infections in vitro and in vivo. Hemolymph concentration of 1%-50% were tested in vitro with doxycycline as controls in paper disc, obtained the lowest hemolymph concentrations with optimum antibacterial activity were 10% with 12±0.62 mm on inhibition zone, optimum blood concentration (10%) was chosen for dextrose-base oral drug. In vivo, 15 mice were infected with 800 microlitre 10 to the power 8 CFU MRSA intraperitoneally and after 3 day infected again with 40 microlitre 10 to the power 8 CFU intravenously. Mice were divided into 3 groups, group I (placebo control), group II (doxycycline), and group III (hemolymph 10%) were given daily for 15 days. Giving of hemolymph 10% for 15 days was able to symptoms of systemic MRSA infections supported by histopathology organ results. Hemolymph 10% has antibacterial activity against MRSA systemic infection in vitro and in vivo.

Kata Kunci : Hemolymph, Scylla serrata, Infeksi MRSA, resistensi