PENGARUH PENAMBAHAN ESSENTIAL OIL NILAM (Pogostemon cablin Benth.) DALAM RANSUM TERHADAP PRODUKSI METAN DALAM FERMENTASI RUMEN SECARA IN VITRO

AHMAD MUBARAK HASAN ASRORI, Ir. Asih Kurniawati, S.Pt., M.Si., IPM.;Prof. Dr. Ir. Lies Mira Yusiati, SU., IPU.

2018 | Skripsi | S1 ILMU DAN INDUSTRI PETERNAKAN

Penelitian ini bertujuan untuk mengetahui pengaruh penambahan essential oil nilam dalam ransum terhadap produksi metan dalam rumen secara in vitro. Perlakuan yang diberikan adalah penambahan essential oil nilam pada ransum yaitu substrat campuran dari rumput gajah, bekatul, dan wheat pollard (60,20,20) dengan kadar level penambahan 0, 25, 50, 75, 100 mg per l medium. Fermentasi dilakukan dengan teknik in vitro produksi gas selama 24 jam pada suhu 39 derajat C dengan ulangan sebanyak 3 kali. Pada akhir fermentasi dilakukan preparasi sampel untuk mengukur nilai pH, kadar VFA, metan, kadar amonia, protein mikrobia dan jumlah protozoa. Data yang diperoleh dianalisis dengan menggunakan Rancangan Acak Lengkap (RAL) Pola Searah. Apabila hasil yang diperoleh berbeda nyata, dilakukan uji Duncan’s New Multiple Range Test (DMRT). Hasil penelitian menunjukkan bahwa penambahan essential oil nilam tidak menunjukkan perbedaan nyata terhadap nilai pH, kadar amonia, protein mikrobia, jumlah protozoa, dan produksi metan. Total VFA meningkat secara nyata (P kurang dari 0,05) dan menurunkan rasio asetat per propionat secara nyata (P kurang dari 0,05).

The aim of this study was to determine the effect of the addition of patchouli essential oil in the ration to in vitro methane gas production of rumen. The treatments given were the addition of patchouli essential oil to the ration that is mixed substrate of Pennisetum purpureum, rice bran, and wheat pollard (60,20,20) with addition level 0, 25, 50, 75, 100 mg per l medium. Fermentation was done by in vitro gas production for 24 hours at 39 degree C with 3 replications. At the end of the fermentation, sample was taken to measure pH values, VFA levels, methane gas, ammonia levels, microbial protein and total protozoa. The data obtained were analyzed using Completely Randomized Design (CRD) one way design. If the results were significantly different, Duncan's New Multiple Range Test (DMRT) was tested. The results showed that pH value, ammonia level, microbial protein, total protozoa, and methane production did not show significant different. Total VFA increased significantly (P less than 0.05) and the ratio of acetate per propionate decreased significantly (P less than 0.05).

Kata Kunci : Minyak Nilam,Essential oil,Produksi metan,Patchouli oil,Essential oil,Methane production