PENGARUH SUPLEMENTASI KALSITRIOL TERHADAP HISTOPATOLOGIK KELENJAR PARATIROID TIKUS DEWASA OSTEOPOROSIS YANG DIINDUKSI PAKAN FOSFOR TINGGI

KEN ROOSMALA DEWI, Dr.drh. Hartiningsih, M.P

2018 | Skripsi | S1 KEDOKTERAN HEWAN

Konsumsi fosfor tinggi menyebabkan hiperfosfatemia, hipokalsemia, hiperparatiroid dan menekan produksi kalsitriol. Penelitian ini dilakukan untuk melihat pengaruh pemberian kalsitriol terhadap histopatologik kelenjar paratiroid tikus dewasa osteoporosis yang diinduksi pakan fosfor tinggi. Sepuluh ekor tikus wistar betina umur delapan minggu dibagi menjadi dua kelompok (OS dan OS40). Dua minggu pasca adaptasi dilakukan induksi osteoporosis, tikus OS dan OS40 diberi pakan fosfor tinggi selama delapan minggu dan air minum aquabidestilata ad libitum. Pasca induksi osteoporosis, kelompok tikus OS diberi pakan standar, sedangkan kelompok tikus OS40 diberi pakan standar ditambah suplemen kalsitriol 40ng/tikus/hari selama lima minggu. Pada akhir penelitian, tikus dieuthanasi, kelenjar paratiroid diambil untuk pemeriksaan histopatologik dengan pengecatan Hematoksilin Eosin (HE). Hasil pemeriksaan histopatologik kelenjar paratiroid tikus OS terjadi peningkatan aktivitas kelenjar paratiroid ditandai banyak sel prinsipal gelap, inti sel mengecil, sitoplasma bervakuola (mengalami hipertrofi), sementara tikus OS40 aktivitas kelenjar paratiroid kembali normal ditandai peningkatan jumlah sel prinsipal terang berinti besar, sitoplasma asidofilik dan sedikit sel hipertrofi. Dari hasil penelitian, disimpulkan bahwa suplementasi kalsitriol 40ng/tikus/hari pada tikus dewasa osteoporosis selama lima minggu menurunkan aktivitas kelenjar paratiroid.

High phosphorus consumption causes hyperphosphatemia, hypocalcemia, hyperparathyroidism and suppresses calcitriol production. This study was conducted to observe the effects of calcitriol administration on the histopathological parathyroid glands of osteoporotic adult rats when induced with a high phosphorus feed. Ten female wistar rats aged eight weeks were divided into two groups (OS and OS40). After two weeks post-adaptation of induced osteoporosis, the OS and OS40 rats were given a high phosphorus feed for eight weeks and drinking water aquabidestilata ad libitum. Post-induction of osteoporosis, the group of OS rats was fed standard feed, while the OS40 rat group was fed standard feed plus a 40ng / rat / day calcitriol supplement for five weeks with drinking water aquabidestilata ad libitum. At the end of the study, the rats was euthanized, the parathyroid glands were removed for histopathological examination and dyed with Hematoxylin Eosin (HE). The results of the histopathological examination of the parathyroid glands of the OS rats showed an increase in activity of the parathyroid glands marked by many dark principal cells, shrinking of the cell nuclei, and cytoplasm of the vacuole (hypertrophy), while in OS40 rats parathyroid gland activity returned to normal. This was marked by an increase in the number of principal bright nuclear cells, acidophilic cytoplasm and fewer hypertrophic cells. From the results of the study, it was concluded that supplementation of calcitriol at 40ng / rat / day in osteoporotic adult rats for five weeks decreased the activity of the parathyroid gland.

Kata Kunci : Kelenjar paratiroid, osteoporosis, fosfor tinggi, kalsitriol / Parathyroid gland, osteoporosis, high phosphorus, calcitriol.