EKSPRESI PROTEIN MATRIX METALLOPROTEINASE-9 PADA TIKUS WISTAR MODEL STROKE

NUR ILMANITA, drh. Sugiyono, M.Sc.

2018 | Skripsi | S1 KEDOKTERAN HEWAN

Stroke adalah salah satu penyebab utama kematian di Indonesia. Stroke iskemik merupakan kasus terbanyak dengan persentase 87% dari seluruh kejadian stroke. Ligasi arteri karotis komunis dapat menginduksi stroke iskemik karena aliran darah menuju otak terhambat sehingga menyebabkan hipoksia melanjut kerusakan jaringan. Kerusakan jaringan akan memicu pengeluaran Matrix metalloproteinase-9 (MMP-9) sehingga dapat dijadikan sebagai biomarker. Ekspresi MMP-9 dapat diukur menggunakan immunohistokimia. Tujuan dari penelitian ini adalah untuk mengetahui ekspresi MMP-9 pada tikus Wistar sebagai hewan model stroke iskemik yang diligasi arteri karotis komunis sinister. Penelitian ini menggunakan 6 ekor tikus Wistar jantan berumur 2 bulan. Tikus dibagi menjadi 2 kelompok yaitu kelompok kontrol dan perlakuan. Tikus kelompok perlakuan diligasi arteri karotis komunis. Tikus perlakuan dipertahankan selama 7 hari sedangkan tikus kontrol dipertahankan selama 30 hari. Nekropsi dilakukan pada tikus. Sampel yang digunakan untuk menganalisis ekspresi MMP-9 pada pewarnaan imunohistokimia adalah cerebellum dan korteks serebri. Antibodi anti MMP-9 digunakan sebagai antibodi primer. Matrix metalloproteinase-9 tikus kontrol dan perlakuan terekspresi pada sel neuron, sel glial korteks serebri dan pada sel purkinje, lapisan molekuler, dan lapisan granular cerebellum. Ekspresi MMP-9 tikus perlakuan mengalami peningkatan 16 kali lipat dari tikus kontrol.

Stroke is a major cause of death in Indonesia. An Ischemic Stroke is the most cases with 87 % percentage occurred. A Stroke Ischemic induced by common carotid artery ligation since the blood flow gets interrupted and induced by hypoxia before makes damage tissue. Tissue damage will trigger MMP-9 as expulsion that can be used as a marker. Immunohistochemistry is using for measured MMP-9. The purpose of this study is to investigate MMP-9 expression in Wistar rats as animal models of ischemic stroke that were ligated in the left common carotid artery. This research used six male Wistar rats aged 2 months. Rats were divided into 2 groups: control and treatment groups. Rats of the treatment group were ligated by a common carotid artery. Rats of the treatment group were maintained for 7 days while control rats were maintained for 30 days. Necropsies were performed on rats. The samples used to analyze Matrix metalloproteinase-9 (MMP-9) expression in immunohistochemical staining were cerebellum and cerebral cortex. Anti-MMP-9 antibodies are used as primary antibodies. Matrix metalloproteinase-9 control and treatment rats, expressed on neuronal cells, glial cells cerebral cortex and in purkinje cells, molecular layers, and granular layer cerebellum. The expression of MMP-9 treatment rats increased 16-fold from control mice.

Kata Kunci : MMP-9, stroke iskemik, ligasi arteri karotis komunis