DETEKSI JOHNE'S DISEASE SECARA SEROLOGI DENGAN METODE ELISA PADA SAPI POTONG PERANAKAN ONGOLE DI KECAMATAN AMBAL, KABUPATEN KEBUMEN

ANASTASIA DIVA P K, Dr. drh. Widagdo Sri Nugroho, M.P.

2018 | Skripsi | S1 KEDOKTERAN HEWAN

Johne's disease merupakan penyakit hewan menular strategis yang disebabkan oleh bakteri Mycobacterium avium subspecies paratuberculosis. Kecamatan Ambal, Kabupaten Kebumen sebagai sentra bibit sapi PO harus bebas dari penyakit hewan menular strategis. Penelitian ini mendeteksi Johne's disease secara serologi dengan metode ELISA dan mengidentifikasi faktor risiko penyebab kejadiannya. Penelitian ini menggunakan serum 36 ekor sapi betina PO berumur minimal dua tahun dengan tanda-tanda Johne's disease berupa diare. Darah sapi diambil melalui vena jugularis menggunakan venoject. Serum darah diuji menggunakan ELISA kit ID Screen���® Paratuberculosis Indirect (IDvet Genetics). Kuisioner terhadap peternak dilakukan untuk mendapat faktor risiko penyebab Johne's disease pada tingkat ternak dan peternak. Faktor risiko tingkat ternak dianalisis menggunakan software SPSS versi 23 (SPSS inc.) dan Microsoft Excel. Hasil ELISA menunjukan enam dari 36 sampel terdiagnosis positif Johne's disease. Pakan liar serta jenis pakan campuran hijauan dan damen memiliki potensi risiko terhadap penyebaran Johne's disease di Kecamatan Ambal, Kabupaten Kebumen.

Johne's disease is included as strategic animal infectious diseases in Indonesia and caused by Mycobacterium avium subspecies paratuberculosis. Ambal sub-district, Kebumen district as PO cattle center thus should free from any strategic infectious animal diseases. The aim of this study were to detect Johne's disease serologically with ELISA method and identified it's risk factors in Ambal sub-district, Kebumen district. Thirty six serum were taken from 36 PO cows which had Johne's disease signs i.e. more than two years old and diarrhea. Cow's blood collected from the jugular vein using venoject. Blood serum tested using ELISA kit ID Screen���® Paratuberculosis Indirect (IDvet Genetics). Farmer interviewed based on questionnaires. The questionnaire contains the risk factors associated to Johne's disease at the level of cattle and farmer. Risk factors were analyzed using software of SPSS version 23 (SPSS inc.) and Microsoft Excel. ELISA result showed six of 36 samples were positively diagnosed by Johne's disease. Weeds and mixed feeding between grass and straw were identified as potential risk to spread the Johne's disease in Ambal sub-district, Kebumen district.

Kata Kunci : Johne's disease, sapi Peranakan Ongole, Ambal, sentra bibit sapi, ELISA