KARAKTERISTIK BIOKIMIA ACINETOBACTER BAUMANNII PENGHASIL EXTENDED SPECTRUM BETA-LACTAMASES (ESBLs) PADA ISOLAT KLINIS DAN LINGKUNGAN RSUP DR. SOERADJI TIRTONEGORO KLATEN

MARIA DIAN TIARASANI, dr. Eggi Arguni, M.Sc, Ph.D, Sp.A; dr. R. Ludhang Pradipta R, M. Biotech, Sp.MK

2017 | Skripsi | S1 KEDOKTERAN

Latar Belakang: Acinetobacter baumannii (A. baumannii) merupakan salah satu patogen yang sering menyebabkan terjadinya infeksi nosokomial serta sudah banyak mengalami resistensi terhadap antibiotik. Faktor lingkungan juga mempengaruhi terjadinya infeksi nosokomial serta dapat menjadi sumber dari penyebaran infeksi tersebut. Tujuan: Mengetahui gambaran A. baumannii penghasil extended spectrum beta-lactamases (ESBL) pada isolat klinis dan lingkungan RSUP Dr. Soeradji Tirtonegoro Klaten. Mengetahui perbedaan karakteristik biokimi antara A. baumannii penghasil ESBL dan non ESBL maupun antara A. baumannii isolat klinis dan lingkungan RSUP Dr. Soeradji Tirtonegoro Klaten. Metode: Penelitian ini menggunakan metode deskriptif eksperimental dengan menggunakan sampel A. baumannii yang diambil dari pasien serta lingkungan sekitar pasien rawat inap Ruang Perawatan Intensif Anak RSUP Dr. Soeradji Tirtonegoro Klaten dan stok isolat bakteri di Departemen Mikrobiologi FK UGM. Proses identifikasi A. baumannnii dilakukan dengan uji oksidase dan alat BD Phoenix. Identifikasi tipe A. baumanni penghasil ESBL menggunakan uji screen disk ESBL + AmpC sedangkan karakteristik biokimiawi didapatkan dengan alat BD Phoenix. Hasil: Empat isolat lingkungan dari 16 isolat klinis dan lingkungan merupakan A. baumannii penghasil ESBL. A. baumannii penghasil ESBL dan non ESBL maupun A.baumannii isolat lingkungan dan isolat klinis mampu memberikan reaksi positif terhadap sitrat dan dekstrosa serta memberikan reaksi negatif terhadap D-manitol, sukrosa, L-rhamnose, ornitin, urea, esculin, arginine, dan lysine. Kesimpulan: Tidak didapatkannya perbedaan karakteristik biokimia antara A. baumannii penghasil ESBL dan A. baumannii penghasil non ESBL maupun pada A. baumannii isolat klinis dan lingkungan. Ditemukannya A baumannii penghasil ESBL pada isolat lingkungan.

Background: Acinetobacter baumannii (A. baumannii) is one of the most common pathogens that cause nosocomial infection and also resistance to antibiotics. Environmental factors affect the occurrence of nosocomial infections and sometimes becomes the source of the spread of the infection. Objective: To determine the description of A. baumannii producing extended spectrum beta-lactamases (ESBL) in clinical and environment isolates of RSUP Dr. Soeradji Tirtonegoro Klaten. To know the differences of biochemical characteristics between A. baumannii ESBL producers and non ESBL producer and also between A. baumannii from clinical isolates and environmental isolates of RSUP Dr. Soeradji Tirtonegoro Klaten. Methods: This study used an experimental descriptive method using A. baumannii's isolates which taken from patients and the environment around inpatient wards PICU RSUP Dr. Soeradji Tirtonegoro Klaten and stock of bacterial isolate in the Department of Microbiology FK UGM. The identification process of A. baumannnii was done by oxidase test and BD Phoenix tool. Identification A. baumannii ESBL producer was determined using ESBL + AmpC screen test while biochemical characteristics were determined with BD Phoenix tool. Results: Four environment isolates of 16 clinical and environment isolates used were A. baumannii ESBL producers. A. baumannii ESBL producers and non ESBL producers also A.baumannii from clinical isolates and environment isolates give positive reaction to citrate and dextrose and give negative reaction to D-mannitol, sucrose, L-rhamnose, ornithine, urea, esculin, arginine, and lysine. Conclusion: No biochemical characteristic difference between A. baumannii ESBL producer and A. baumannii non ESBL producers also A. baumannii from clinical isolates and environment isolates. A baumannii ESBL producer were found in environment isolates

Kata Kunci : Acinetobacter baumannii, ESBL, biochemical characteristics, ESBL + AmpC screen test.