EFEK SUPLEMENTASI ALPHA LIPOIC ACID (ALA) DAN KAFEIN TERHADAP STATUS STRES OKSIDATIF HEPAR TIKUS DIABETES

KHOIRUL RISTA ABIDIN, dr.Andreanyta Meliala., Ph.D., AIFM.; Dr.dr.Sri Lestari Sulistiyo Rini., M.Sc

2018 | Tesis | S2 Ilmu Kedokteran Dasar dan Biomedis

Latar belakang : Diabetes merupakan penyakit metabolik yang ditandai dengan hiperglikemia sehingga memicu stres oksidatif. Alpha Lipoic Acid (ALA) dapat digunakan untuk menekan peningkatan stres oksidatif. Sedangkan suplementasi kafein dapat digunakan untuk mencegah stres oksidatif pada kondisi normal. Penelitian ini dilakukan untuk mempelajari efek suplementasi kafein dan ALA terhadap status stres oksidatif pada organ hepar tikus diabetes dengan melihat kadar Malondialdehid (MDA) dan Glutathion Peroksidase-1 (GPx-1) sebagai parameter. Metode : Penelitian ini menggunakan desain eksperimen murni dengan rancangan post test only with control group design. Subjek penelitian sebanyak 30 ekor Wistar jantan dibagi menjadi 5 kelompok : Normal (N), Diabetes (D), Diabetes+Kafein (DK), Diabetes+ALA (DA), Diabetes+Kafein+ALA (DKA). Kelompok diabetes diinduksi menggunakan Streptozotocin (STZ) 60 mg/kg berat badan. Suplementasi kafein (60 mg/kg berat badan) dan ALA (50 mg/kg berat badan) diberikan setiap hari selama 6 minggu. Uji kadar MDA dan GPx-1 menggunakan teknik ELISA. Hasil : Tidak ditemukan perbedaan bermakna pada kadar MDA (72,56+-7,82) dan GPx-1 (80,91+-15,37) hepar tikus diabetes dengan suplementasi kafein dan ALA dibandingkan kadar MDA (95,15+-7,98) dan GPx-1 (66,81+-10,19) hepar tikus diabetes tanpa kafein dan ALA. Kesimpulan : Penelitian ini tidak menunjukkan perbedaan bermakna (p>0,05) pada kadar MDA dan GPx-1 hepar tikus diabetes dengan kafein dan ALA dibandingkan kadar MDA dan GPx-1 hepar tikus diabetes tanpa kafein dan ALA.

Research background: Diabetes is one of the metabolic diseases marked by hyperglycemia, triggering oxidative stress. To prevent the increase of the oxidative stress, we can utilize Alpha-lipoic Acid (ALA). Besides, to prevent the oxidative stress from occurring during the normal condition, we can use caffeine as a supplement. This research aims to study the effects of caffeine and ALA as supplements on the status of oxidative stress in the liver of diabetic rats, conducted by observing the Malondialdehyde (MDA) and Glutathione Peroxidase-1 (GPx-1) levels as a parameter. Research methods: This researchers applied a true experimental research design by employing a posttest only with control group design. The research subject consisted of thirty male Wistar rats that were divided into five groups: Normal (N), Diabetes (D), Diabetes + Caffeine (DC), Diabetes + ALA (DA), and Diabetes + Caffeine + ALA (DCA). The diabetic rats were supplemented with Streptozotocin (STZ) 60 mg/kg body weight. Caffeine (60 mg/kg body weight) and ALA (50 mg/kg body weight) were supplemented every day for six weeks as supplements. The MDA and GPx-1 levels were tested by employing the ELISA technique. Research findings: There was no significant difference between MDA (72.56+-17.82) and GPx-1 (80.91+-15.37) levels in the liver of diabetic rats supplemented with caffeine and ALA and the MDA (95.91+-7.98) and GPx-1 (66.81+-10.19) levels in the liver of diabetic rats without being supplemented with caffeine and ALA. Conclusion: There was no significant difference (p > 0.05) between MDA and GPx-1 levels in the liver of diabetic rats supplemented with caffeine and that in the liver of diabetic rats without being supplemented with caffeine and ALA.

Kata Kunci : diabetes mellitus, kafein, ALA, MDA, GPx-1