OPTIMASI PRODUKSI KITINASE Streptomyces sp. PB-2 PADA BERBAGAI pH MEDIUM DAN SUHU INKUBASI

FATKHANA INDAH K, Indun Dewi Puspita, S.P.,M.Sc., Ph.D.

2018 | Skripsi | S1 TEKNOLOGI HASIL PERIKANAN

Penelitian ini bertujuan untuk mengoptimasi bakteri Streptomyces sp. PB-2 dalam produksi kitinase pada berbagai pH medium (5, 6, 7, 8, dan 9) dan suhu inkubasi (30oC, 40oC, dan 50oC). Parameter yang diuji meliputi produksi kitinase (U/ml) dan konsentrasi N-asetilglukosamin (NAG) dalam medium (�¼g/ml) serta pertumbuhan bakteri (log CFU/ml). Produksi kitinase dan konsentrasi NAG dianalisis secara kuantitatif dengan metode kolorimetri. Sedangkan pertumbuhan bakteri Streptomyces sp. PB-2 diuji menggunakan metode TPC (Total Plate Count). Hasil penelitian menunjukkan bahwa produksi kitinase oleh bakteri Streptomyces sp. PB-2 optimum pada pH 7 (0,0021 U/ml) di hari ke-3 inkubasi dan optimum pada suhu 30oC (0,0017 U/ml) di hari ke-6 inkubasi. Evaluasi hubungan antara produksi kitinase dan pertumbuhan bakteri Streptomyces sp. PB-2 menunjukkan keterkaitan atau disebut Growth Associated Product.

This study aimed to optimize chitinase production by Streptomyces sp. PB-2 at various pH medium (5, 6, 7, 8, and 9) and various incubation temperature (30oC, 40oC, and 50oC). Parameters examined included chitinase production (U/ml), the concentration of N-acetylglucosamine (NAG) in medium (�¼g/ml), and bacteria growth (log CFU/ml). Chitinase production and the concentration of NAG were analyzed quantitatively by colorimetric method, while the bacteria growth were measured by TPC (Total Plate Count) method. The result shows that the chitinase production by Streptomyces sp. PB-2 was optimum at pH 7 (0,0021 U/ml) on the day-3 incubation and optimum temperature at 30oC (0,0017 U/ml) on the day-6 incubation. Evaluation of the interaction among chitinase production and growth of Streptomyces sp. PB-2 shows that they were related one another or called Growth Associated Product.

Kata Kunci : Kata kunci: Optimasi, kitinase, Streptomyces sp. PB-2, pH medium, suhu