SINTESIS FILM KOMPOSIT KITOSAN-TiO2-Ag SEBAGAI MATERIAL ANTIBAKTERI; SYNTHESIS OF CHITOSAN-TiO2-Ag COMPOSITE FILM AS ANTIBACTERIAL MATERIAL

MAMAHIT, FANDY EDWARD, Eko Sugiharto

2016 | Skripsi | FMIPA

Synthesis of chitosan-TiO2-Ag composite film has been carried out. The aim of this research are to study the effect of adding Ag for antibacterial properties and the effect of weight ratio of TiO2-Ag to chitosan toward mechanical properties, which are tensile strength and elongation properties of chitosan-TiO2-Ag composite film. The method for synthesizing chitosan-TiO2-Ag composite film was conducted by solvent casting method. First step of the synthesis was started with the preparation of TiO2 through sol-gel method using TTIP (Titanium (IV) isopropoxide) as precursor. After TiO2 powder was formed, modification TiO2 with Ag was performed by photoreduction method using UV light. Next, TiO2-Ag powder was diluted in 2% (w/v) chitosan solution and the mixed solution was casted in stainless steel container (9 x 13 cm). The solvent was left to evaporate until the film was formed. The films were prepared using various weight ratio of Ag to chitosan of 1, 2, 3, 4, and 5%, and various weight ratio of TiO2-Ag to chitosan of 10, 15, 20, 25, and 30%. In addition, preparation of chitosan, chitosan-TiO2, and chitosan-Ag film also carried out as comparison material. The characterization was perfomed using X-Ray Difractrometer (XRD), Fourier Transform Infra Red (FTIR) Spectrophotometer, and Diffuse Reflectance UV-Vis (DR-UV) Spectrophotometer. Moreover, the antibacterial properties was evaluated with E. coli bacteria using plate count method and the mechanical properties was tested using Universal Testing Machine. The result of this research were antibacterial and mechanical properties. For antibacterial case, it was showed that increasing Ag in the composite not only increased the antibacterial properties of the composite but also effective for inhibiting E. coli bacteria in 3, 4, and 5% of Ag. The result of mechanical properties were tensile strength and elongation properties. The addition of weight ratio of TiO2-Ag to chitosan increasing the tensile strength and decreasing elongation properties.

Kata Kunci : chitosan-TiO2-Ag composite film, antibacterial properties, mechanical properties.