FRAKSINASI PROTEIN pI 5 DAN pI 8 BIJI CARICA (Carica pubescens) DENGAN EKSTRAKSI BASA SERTA KAJIANNYA SEBAGAI BAHAN FORTIFIKASI PANGAN; FRACTINATION OF PROTEIN pI 5 AND pI 8 FROM CARICA SEED (Carica pubescens) BY ALKALINE EXTRACTION AND STUDY AS FOOD FORTIFICATION MATERIAL

HIDAYATULLAH, MUHAMMAD SYARIF, Tri Joko Raharjo

2016 | Skripsi | FMIPA

Fractination of proteins pI 5 and pI 8 of carica (Carica pubescens) seeds and study for food fortification have been done. This research aims to determine the nutrient content of the seeds, extract of the protein at alkaline pH, fractionate using pI 5 and pI 8, and identify the essential amino acid content. The determination of nutrition content carica seed was done by proximate analysis. The water content was determined by gravimetric, ash content was determined by ashing sample at 550 °C, fat content was determined by Soxhletation, protein content was determined by Kjeldhal method and carbohydrates was calculated by different. Fatty acids contained in carica seeds oil was analyzed by using GC-MS through derivatization BF3-methanol. Protein was extracted from fat-free samples in alkaline conditions at pH 12. Fractination of protein was carried out by isoelectric precipitation at pI 5 and pI 8. Amino acid was analyzed by using reverse phase HPLC with flourescence detector. The carica (Carica pubescens) seeds contain 4.64 ± 0.15 % of water, 4.87 ± 0.07 % of ash, 35.38 ± 0.71 % of fat, 28.28 ± 1.01 % of protein and 26.83 % of carbohydrates. Carica seeds oil contains 71.08 % of oleic acid, 15.62 % of palmitic acid, 9.70 % of linoleic acid and 3.59 % of stearic acid. Fraction pI 5p and pI 8 (first precipitation) was recovered 9.01 % and 87.41 %. Fraction pI 5s (second precipitation remaining of supernatant fraction pI 8) was recovered 77.14 %. The essential amino acids contain 44.41 % of fraction pI 5s, 35.44 % of fraction pI 5p, and 0.63 % of fraction pI 8. Fraction pI 5 is to be used protential as food fortification material. Fraction pI 5s are fulfilling essential amino acids requirement in the regulation FAO/WHO 1985 for adult and child preschool while fraction pI 5p only for adult.

Kata Kunci : Carica (Carica pubescens) seeds, fatty acids, alkaline extraction, isoelectric precipitation, fortification