STUDI PELEPASAN ?–TOKOFEROL DARI FILM KOMPLEKS POLIELEKTROLIT KITOSAN/PEKTIN DAN POTENSINYA SEBAGAI ANTIOXIDANT PACKAGING; STUDY OF ?–TOCOPHEROL RELEASE FROM CHITOSAN/PECTIN POLYELECTROLYTE COMPLEX FILM AND ITS POTENTIAL AS AN ANTIOXIDANT PACKAGING

HAPSARI, AULIA RATRI, Dwi Siswanta

2016 | Disertasi | FMIPA

conducted. Some factors that affected the release of ?–tocopherol were studied, including composition of PEC chitosan/pectin, concentration of Tween–80 and concentration of ?–tocopherol. PEC chitosan/pectin was synthesized at pH 4 and hydrophobic antioxidant ?–tocopherol was added into hydrophilic PEC chitosan/pectin by adding non–ionic surfactant Tween–80. The prepared films were characterized using FTIR spectrophotometer. In vitro release of ?–tocopherol was investigated for 10 days in ethanol 96% (v/v) as fatty food simulant. The concentrations of ?–tocopherol were determined spectrophotometrically. The result of FTIR showed that ionic interaction occured between ionized amino groups of chitosan (–NH3+) and ionized carboxyl acid groups (–COO–) of pectin, leading to the formation of a polyelectrolyte complex (PEC). This research showed that composition of PEC chitosan/pectin, concentration of Tween–80 and concentration of ?–tocopherol affected the release of ?–tocopherol. Hydrophilicity of film increased with the rising content of pectin in PEC and concentration of Tween–80. This improvement in the hydrophilicity also leads to the increased of release rate of ?–tocopherol. The increase of ?–tocopherol concentration also promoted an increase of release rate of ?–tocopherol due to plasticizer effect in the films. Release mechanism of ?–tocopherol from PEC chitosan/pectin films followed Korsmeyer–Peppas model which indicated that the release mechanism was mainly controlled by Fickian diffussion process. The result of the performed DPPH assay showed that films exhibited high antioxidant activity. The release profile of all films exhibited an intial burst effect followed by sustain release over 10 days. Therefore, this films can be promising as an antioxidant packaging.

Kata Kunci : PEC chitosan/pectin, ?–tocopherol, release, antioxidant, packaging