QUANTITATIVE ANALYSIS OF SURFACE PLASMON RESONANCE BASED BIOSENSOR RESPONSE FOR DETECTING BOVINE AND PORCINE GELATIN DIFFERENCE

WARDANI, DEVY PRAMUDYAH, Kamsul Abraha

2016 | Disertasi | FMIPA

Gelatin is a biopolymer derived from collagen, mainly from pig and cow, that is widely used in food and pharmaceutical industries. Due to some religion and health issues regarding the gelatin consumption which is extracted from certain species, it is necessary to establish a robust, reliable, sensitive and simple quantitative method to detect gelatin from different sources. To the best of our knowledge, there has not been a gelatin differentiation method based on optical sensor that could detect gelatin from different species quantitatively. Surface plasmon resonance (SPR)-based biosensor is known to be a sensitive, simple and label free optical method for detecting biomaterials that is able to do quantitative detection. We had utilized SPR-based biosensor to detect the difference between bovine and porcine gelatin in various concentration, from 0% to 10% (w/w). We used two methods in this study, i.e. observing SPR phenomena of gelatin samples by using the same prism/Au system and by using a different system for each gelatin sample. Here, we report the ability of SPR-based biosensor to detect the difference between both gelatins quantitatively, its sensitivity towards the gelatin concentration change, its reliability, its limit of detection (LOD), and its limit of quantification (LOQ) of the sensor. The models of the sensor responses show that SPR-based biosensor had nonlinear response in detecting gelatin concentration change. The sensor’s LOD and LOQ towards bovine gelatin concentration were 0.38% and 1.26% (w/w), while towards porcine gelatin concentration were 0.66% and 2.20% (w/w), respectively, by using the first method. From the second method, the LOD and the LOQ of the sensor were 0.22% and 0.74% (w/w), respectively. The results show that SPR-based biosensor is a promising tool for detecting gelatin from different raw materials quantitatively.

Kata Kunci : surface plasmon resonance (SPR), biosensor, bovine gelatin, porcine gelatin, quantitative detection.