UJI AKTIVITAS ANTIBAKTERI, ANTIMALARIA, DAN IDENTIFIKASI SENYAWA EKSTRAK KARANG LUNAK Lobophytum sp. DARI PERAIRAN PULAU SELAYAR, SULAWESI SELATAN; THE ANTIBACTERIAL ACTIVITY TEST, ANTIMALARIAL ASSAY, AND IDENTIFIYING THE EXTRACT COMPOUNDS OF SOFT CORAL Lobophytum sp. FROM SELAYAR ISLAND, SOUTH SULAWESI

Widhiana, Meilia Rahma, Respati Tri Swasono

2016 | Skripsi | FMIPA

The aims of this research were to identifying bioactive compounds and to determine the antibacterial and antimalarial activities of extract of soft coral Lobophytum sp. from Selayar Island, South of Sulawesi. Isolation was performed by maceration using a mixture of methanol:dichloromethane (1:1 v/v). The extract was then partitioned by n-hexane, ethyl acetate, n-butanol. Fraction from each samples were tested for their antibacterial activities using agar diffusion method and antimalarial activities using inhibition of hem polymerization method. The highest fraction of antibacterial and antimalarial activities were then identified by Liquid Chromatography-Mass Spectrometry (LC-MS). The ethyl acetate fraction of Lobophytum sp. from 3 and 6 meters depth has moderate and strong inhibition against the bacteria Bacillus subtilis with the inhibition of 9.375 and 11.675 mm respectively. Ethyl acetate fraction of Lobophytum sp. 3 meters depth has IC50 value of 11.718 ppm and the fraction of butanol from 6 meters depth has IC50 value of 11.364 ppm, showed that these fractions have antimalarial activity because it has a lower IC50 value than chloroquine IC50 value that is equal to 13.073 ppm. The ethyl acetate fraction might contain menelloid E, 2-hydroperoxysarcophine, and lobophyton O. The butanol fraction might contain lobophyton Q and durumolide O.

Kata Kunci : Lobophytum sp., antibacterial , antimalarial, agar diffusion, hem.