PEMBUATAN NANOFIBER POLI (VINIL ALKOHOL)/KITOSAN (CS) DENGAN METODE ELEKTROSPINNING YANG TERTAUT SILANG DENGAN GLUTARALDEHIDA SEBAGAI MEMBRAN ANTIBAKTERI; FABRICATION OF POLY (VINYL ALCOHOL)/CHITOSAN (CS) ELECTROSPUN NANOFIBER CROSSLINKED WITH GLUTARALDEHYDE FOR ANTIBACTERIAL MEMBRANE

Loi, Fames Humala, Ahmad kusumaatmaja

2016 | Skripsi | FMIPA

Nanofibers Chitosan/poly (vinyl alcohol) have been successfully produced by electrospinning method using solution of Chitosan (4 wt% and 5 wt% ) and poly (vinyl alcohol) (PVA) 20 wt% with ratio 30:70 and 50:50 in 2% acetid acid solvent. The morphology of nanofibers before and after submersion in Glutaraldehyde was investigated using Scanning Electron Microscopy (SEM). Electrospun nanofiber was submersed at temperature room for 24 hours. After submersion, there was increase in the diameter of nanofiber PVA/CS membrane. Before submersion, the average diameter of nanofiber PVA/CS 4 wt% 70:30 is (160 ± 20) nm and 50:50 is (120 ± 30) nm while nanofiber PVA/CS 5 wt% 70:30 is (300 ± 60) nm and 50:50 is (240 ± 30) nm. After submersion, the average diameter of nanofiber PVA/CS 4 wt% 70:30 became (220 ± 30) nm and 50:50 became (200 ± 30) nm while nanofiber PVA/CS 5 wt% 70:30 became (340 ± 40) nm and 50:50 became (250 ± 40) nm. As results showed the increases in the average diameter of nanofibers with increasing the concentrasion of Chitosan. Nanofiber PVA/CS 5 wt% with ratio 70:30 has highest average diameter (300 ± 60) nm while PVA/CS 4 wt% with ratio 50:50 has the smallest average diameter (120 ± 30) nm. The increasing of PVA ratio in the solution cause higher swelling ratio. Nanofiber PVA/CS 5 wt% with ratio 70:30 has highest swelling ratio 1450%. For bacterial treatment using patogen bacterial Escherichia coli, nanofiber PVA/CS 5 wt% with ratio 70:30 has antibacterial characteristic but low bacterial resistance.

Kata Kunci : Chitosan, Poly (vinyl alcohol), nanofiber, Glutaraldehyde, swelling ratio, antibacterial, electrospinning