IDENTIFIKASI, UJI TOKSISITAS, DAN UJI ANTIMALARIA FRAKSI ETIL ASETAT SPONS Theonella sp. DARI PERAIRAN BARRANG LOMPO, SULAWESI SELATAN; IDENTIFICATION, TOXICITY TEST, AND ANTIMALARIAL ASSAY OF ETHYL ACETATE FRACTION OF Theonella sp. SPONGE FROM BARRANG LOMPO SEA, SOUTH SULAWESI

Istiqomah, Anisa ayu, Respati Tri Swasono

2015 | Skripsi | FMIPA

Identification, toxicity test, and antimalarial test of bioactive compounds from Theonella sp. collected in Barrang Lompo sea, South Sulawesi were done. Extraction was performed by maceration with a mixture of methanol:dichloromethane (3:2 v/v). The dichloromethane extract was then concentrated and partition by liquid-liquid extraction in ethyl acetate:water (1:1 v/v). Ethyl acetate fraction was separated by column chromatography with step gradient polarity system of eluent. Eluents used were n-hexane, n-hexane: dichloromethane, dichloromethane, dichloromethane: ethly acetate, ethyl acetate, ethyl acetate:methanol, and methanol with ratio of mixture were 9:1, 8:2, 7:3, 5:5, 3:7, 2:8, 1:9 v/v. The toxicity of crude extract and fractions of column chromatography were tested using Brine Shrimp Lethality Test (BSLT) method. Fractions which has higher toxicity then tested using hematin polymerization inhibition method for antimalarial test. Fractions containing antimalarial activity were analyzed by Liquid Chromatography – High Resolution Electrospray Ionization Mass Spectrometry (LC-HRESIMS). Crude extract obtained from the extraction is 1.01 g and the value of Lethal Concentration 50% (LC50) is 1.18 ppm. Fraction II, III, and VIII showed high toxicty at single test concentration of 10 ppm. Hematin polimerization inhibition test revealed the fraction II, III, and chloroquin have IC50 values of 0.733; 0.369; and 3.230 mg/mL, respectively. LC-HRESIMS analysis of the fraction II and III shows that fraction II might contains spirotoamide B, rhodopeptin C1, 10-apo-astaxhantinal, tandyukisin B, hapaioside, and fraction III might contains cylindricine (D, E), maltophilin, rhodopeptin C1, theonellasterol I, ?- cryptoxanthin, swinhosterol C.

Kata Kunci : Sponge Theonella sp.; Brine Shrimp Lethality Test; hematin polymerization inhibition method; LC-HRESIMS