DISTRIBUSI KURKUMIN DAN KURKUMINOID PADA EMPU DAN ANAKAN RIMPANG Curcuma mangga Val. SERTA AKTIVITASNYA SEBAGAI ANTIOKSIDAN ALAMI; DISTRIBUTION OF CURCUMIN AND CURCUMINOID FROM MOTHER AND FINGER RHIZOMES OF Curcuma mangga Val. AND IT’S ACTIVITY AS A NATURAL ANTIOXIDANT

Eunikhe, Y Putri, Endang Tri Wahyuni

2015 | Skripsi | FMIPA

The studies of distribution curcumin and curcuminoid namely demethoxycurcumin and bisdemethoxycurcumin on mother and finger rhizomes of Curcuma mangga Val. and its activity as a natural antioxidant have been carried out. The study began with the isolation of curcumin and curcuminoid using the maceration extraction method with 95% of ethanol for 24 hours. Curcumin and curcuminoid have been identified by using TLC method with the optimized eluent, and its purities measured by using TLC Scanner. Curcumin and curcuminoid were purified using Preparative Thin Layer Chromatography (PTLC) method and the results analyzed by HPLC and identified its structure by using FTIR. Curcumin and curcuminoid were tested as a natural antioxidant activity using DPPH radical scavenging method. The result of the study showed that there were differences in purities of curcumin and curcuminoid on mother and finger rhizomes of Curcuma mangga Val. of 1.67% and 2.89%, respectively. PTLC purification method can be used to purified the curcumin compound based on the analysis by HPLC and identification using FTIR, but it did not work on demethoxycurcumin and bisdemethoxycurcumin. The antioxidant activity (IC50) amount of curcumin and curcuminoid obtained in the mother and finger rhizomes of Curcuma mangga Val. were 78.54 ?g/mL and 71.03 ?g/mL, respectively. The order of antioxidant activity for the individual components of the mother and finger rhizomes were curcumin>demethoxycurcumin>bisdemethoxycurcumin. The best antioxidant activity results were 63.68 ?g/mL and 69.82 ?g/mL as IC50 number of the isolated curcumin mother and finger rhizome

Kata Kunci : Curcuma mangga Val.; curcumin; curcuminoid; antioxidant