PENGEMBANGAN METODE ANALISIS SURFAKTAN ANIONIK NATRIUM DODESIL BENZENA SULFONAT (SDBS) DALAM IKAN AIR TAWAR SECARA SPEKTROFOTOMETRI UV-Vis MENGGUNAKAN ACRIDINE ORANGE; ANALYTICAL METHOD DEVELOPMENT OF ANIONIC SURFACTANT SODIUM DODECYL BENZENE SULFONATE (SDBS) IN FRESH WATER FISH BY UV-Vis SPECTROPHOTOMETRY USING ACRIDINE ORANGE

Ratri, Monica C, Adhitasari Suratman

2015 | Disertasi | FMIPA

Analytical method development of anionic surfactant sodium dodecyl benzene sulfonate (SDBS) in fresh water fish by UV-Vis spectrophotometry using acridine orange has been conducted. The aims of this research are to determine the optimum conditions of analysis, the effect of protein and coexisting ion in analysis, the validation parameters, the effect of SDBS concentration in the pond on SDBS accumulation in catfish, and the concentration of SDBS in fish from local markets. This study was divided into two steps, isolation of SDBS in the fish with Soxhlet extraction and analysis of SDBS. The analysis of SDBS is based on the formation of ion pair between DBS and AO. The results showed that the analysis can be performed at 499 nm, using ethanol as AO solvent, mole ratio of DBS AO 2:1, and pH 2.97. Interference came from protein, Ca2+ and Mg2+. The parameters of validation method have good acceptability as linearity (R2) of 0.997, limit of detection of 0.0343 mg/L and limit of quantification of 0.104 mg/L, precicision of 0.382 - 1.78%, sensitivity of 4.64 x 104 L mol-1cm-1, and accuracy of 82.11-100.3%. SDBS concentration in the fish tissue increases with the increase in SDBS concentration in cultivating water. The concentration of SDBS in catfish, red tilapia fish, and carp from local markets were 0.0012 to 0.0029 mg/g, 0.0038 to 0.0077 mg/g and 0.0008 to 0.0026 mg/g, respectively.

Kata Kunci : validation method; SDBS; acridine orange; fish