SCREENING OF NOVEL SECONDARY METABOLITES FROM ENDOPHYTIC FUNGI OF TROPICAL FOREST
Ariefta, Nanang R, Sabirin Matsjeh
2015 | Disertasi | FMIPAThe screening of novel secondary metabolites from tropical forest endophytic fungi has been done. This study aimed to screen potential endophytic fungi from plants of tropical forest origin, which possessed ability to produce novel secondary metabolites, based on chemical profile on TLC analysis. The screening was supported by biological activity test. This research was done also to isolate and determine structure of the secondary metabolites. The fermentation used solid static culture media, chromatography separation for the purification, and spectroscopy analysis for structure determination. Isolation of endophytic fungi from unidentified dead plants from Thailand area, yielded 275 fungi. Screening of 275 fungi were conducted based on the compound profiles in TLC analysis, supported by antimicrobial and seed germination activity. This gained 39 potential fungi that have the ability to produce novel compounds. From 39 potential fungi, two fungi with label T5 and T13 were chosen for further study. Fungi T5 identified as Aspergillus stellatus. Fermentation, extraction, isolation, and structure determination of metabolites produced by T5 and from those processes obtained eight known compounds that were shamixanthone, tajixanthone hydrate, emerixanthone A, 14-hydroxytajixanthone-25-acetate, 14-metoxytajixanthone-25-acetate, dihydroterrein, terrein, and asteltoxin. Fungi T13 identified as Fusarium solani. First fermentation, extraction, isolation, and structure determination of metabolites from T13 produced seven compounds that were two known compounds de-o-methyllasiodiplodin and 3,5-dihydroxy-7-(8-hydroxynonyl) benzoate, three novel compounds were ?-resorcylic acid derivative 1, 2, and 5, one novel compound was the lasiodiplodin derivative 3, and one novel compound was spirobisnaphthalene derivative 4. From the upscale fermentation of T13, the extraction, isolation, and structure determination of metabolites, obtained two known compounds 1233B and chrysanthone B, one novel compound 6 with ?-lactone ring, and one known compound derivative, methyl ester-6. The discovery of those novel secondary metabolites is one of the evidence that cultures of endophytic fungi are proved to be a potential source of novel compounds.
Kata Kunci : screening; endophytic fungi; isolation; novel compounds, Aspergillus stellatus; Fusarium solani
