PEMBUATAN, KARAKTERISASI DAN STUDI PELEPASAN OBAT IN VITRO KURKUMIN TERSALUT NANOPARTIKEL KITOSAN-PEKTIN DENGAN DAN TANPA ASAM SITRAT SEBAGAI AGEN TAUT SILANG

PANDU SIAGAWATININGTYAS, Dwi Siswanta

2015 | Disertasi | FMIPA

Curcumin is a yellow natural polyphenol extracted from turmeric (Curcuma longa L.) and has a potent anticancer properties that was demonstrated in various human cancer cells. However, the widespread clinical application of this efficient agent in cancer and other diseases has been limited by its poor aqueous solubility and bioavailability. In this study, synthesis of curcumin loaded chitosan-pectin nanoparticles with and without citric acid as crosslink agent and their evaluation on in vitro drug release study have been done. Chitosan-pectin-curcumin nanoparticles (chit-pec-cur nps) and chitosan-pectin-curcumin-citric acid nanoparticles (chitpec- cur-ca nps) were prepared by gelation ionic method. The characterization for structure, size and morphology characterization of nanoparticles were investigated by Fourier Transform Infra Red (FT-IR), Transmission Electron Microscopy (TEM), and Scanning Electron Microscopy (SEM). The profile, kinetics and mechanism for in vitro drug release from the nanoparticles were studied under simulated physiological conditions for different incubation periods during 98 hours. The FT-IR analysis resulted that potential interactions was revealed among the constituents in nanoparticles. The particles of chit-pec-cur nps and chit-pec-cur-ca nps were spherical shape with average size of 45 and 230 nm, respectively. Entrapment efficiency (%) of curcumin in chit-pec-cur nps and chit-pec-cur-ca nps were 40 dan 56, respectively. Drug release kinetics for both products tend followed Power law models with a rate constants for chit-pec-cur nps and chit-pec-cur-ca nps were 4.634 hours-0,580 and 2.339 hours-0,716, respectively. Drug release mechanism from the matrix followed anomalous-transport (non-Fickian) mechanism.

Kata Kunci : curcumin; citric acid; pectin; nanoparticles; in vitro drug release.