DISSIPATION PROFILE OF AZOXYSTROBIN IN SOIL AS INFLUENCED BY TEMPERATURE, SOLAR RADIATION, AND SOIL CHARACTERISTIC; PROFIL DISIPASI AZOXYSTROBIN DALAM TANAH YANG DIPENGARUHI OLEH SUHU, RADIASI MATAHARI, DAN KARAKTERISTIK TANAH
Purnama, Indra, Bambang Rusdiarso
2015 | Disertasi | FMIPA UGMDissipation studies of azoxystrobin in soil were analyzed by a HPLC. Extractions of the soil were done by liquid-liquid extraction (LLE) with two methods, Environmental Protectian Agency (EPA) method (classical) and Quick, Easy, Cheap, Effective, Rugged, and Safe (QuEChERS) method (modern) through the experimental batch system. Dissipation of azoxystrobin in soil by controlling the temperature was done at 5, 20, and 35 oC. Influence of solar radiation on dissipation rate of azoxystrobin in soil was studied in two different conditions, in light conditions (exposure to sunlight) and dark reactions (in a dark closet). Azoxystrobin dissipation rate in different characteristic of soil was studied in this study. QuEChERS method showed better performance than the EPA method. Analysis method of azoxystrobin has been validated using HPLC by means, accuracy, precision, sensitivity, and linearity. Limit of detection (LOD) and limit of quantification (LOQ) were 0.02 mg/Kg and 0.05 mg/Kg, respectively. The relative recoveries of the azoxystrobin were acceptable (81.7-107.9%). The dissipation rate of azoxystrobin in soil, under the control of the temperature at 35 oC (0.0146/day, t½ = 47 days) was faster than the temperature at 20 oC (0.0129/day, t½ = 54 days) and at 5 oC (0.0072/day, t½ = 96 days). The dissipation rate of azoxystrobin in light conditions (0.2018/day, t½ = 3.4 days) was faster than in dark conditions (0.0912/day, t½ = 7.6 days). Dissipation of azoxystrobin in soil as influenced by temperature and solar radiation were significantly different (P<0.05). Different type of microorganism in two soils characteristic was assumed to cause differences of dissipation rate of azoxystrobin in soil.
Kata Kunci : azoxystrobin; temperature; solar radiation; extraction; QuEChERS; chromatography
