SINTESIS PIRAZOLINA DARI NITROVERATRALDEHIDA DAN KLOROASETOFENON SERTA UJI AKTIVITAS SEBAGAI ANTIBAKTERI;SYNTHESIS OF PYRAZOLINE FROM NITROVERATRALDEHYDE AND CHLOROACETOPHENONE WITH ACTIVITY TEST AS ANTIBACTERIAL
Ichsan, Fikri Rachmadani, Wahyuningsih, Tutik Dwi
2015 | Skripsi | FMIPA UGMSynthesis of pyrazoline from nitroveratraldehyde and chloroacetophenone with activity test as antibacterial has been investigated. The first step, nitration of veratraldehyde was conducted with a mixture of nitric acid and sulfuric acid at 5 ?C. Then, aldol condensation was performed by reacting nitroveratraldehyde with chloroacetophenone produced chalcone. Synthesis of pyrazolines was performed by reacting chalcone and phenylhydrazine under reflux method for 4 h. The product was analyzed by FTIR, GCMS, 1H and 13C-NMR spectrometers. Antibacterial test was conducted by well-method against Gram positive (Staphylococcus aureus, Bacillus cereus, Bacillus subtilis) and Gram negative (Eschericia coli dan Shigella flexneri) bacteries, with tetracycline as positive control and dimethyl sulfoxide (DMSO) as negative control. The result showed that nitration of veratraldehyde yield in 70.17%, chalcone and pyrazoline have been produced in 14.85% and 33.84% yield, respectively. Antibacterial test showed that pyrazoline has a best potential as antibacterial at 500 ppm against bacteria Staphylococcus aureus with DDH value 6.75 mm and at 1000 ppm against bacteria Bacillus subtilis with DDH value 2.25 mm.
Kata Kunci : pyrazoline; chalcone; nitroveratraldehyde; antibacteria; DDH
