KAJIAN SIFAT OPTIK NANOFIBER POLIANILIN TERDOPING HCl DAN POTENSINYA SEBAGAI BAHAN AKTIF BIOSENSOR BERBASIS SURFACE PLASMON RESONANCE (SPR); STUDY ON THE OPTICAL PROPERTIES OF POLYANILINE NANOFIBERS DOPED BY HCl AND THEIR POTENTIAL AS ACTIVE MATERIALSFOR SURFACE PLASMON RESONANCE (SPR) BASED BIOSENSORS

SITTI NURRAHMI, Kamsul Abraha

2014 | Disertasi | PROGRAM STUDI S2 ILMU FISIKA

Polyaniline nanofibers have been successfully fabricated with variation of dopant concentration to see it’s potential as active materials for Surface Plasmon Resonance (SPR) based biosensors. The Study began with synthesing the monomer anilin to generates polyaniline nanofibers by using interfacial polymerization methods with dopant Hydrochloric Acid (HCl) then varies the concentration until 3 M. The results of Scanning Electron Microscope (SEM) showed that the greater the HCl concentration used, the smaller the diameter polyaniline nanofibers and has clear fibers. The results of Fourier Transform Infrared spectroscopy (FTIR) showed that there has been a shift in the peak position of vibration characteristics from a standard curve for each HCl concentration used. The result of UV-Visible spectroscopy showed that the greater the HCl concentration used, the smaller the values of absorbance. The results of Kramers-Kronig relationship using Python program, the effect of variations concentration HCl for refractive index (n) and extinction coefficient (?) values at wavelength of He-Ne laser (? = 632.8 nm) have a tendency to decreasing the values of n and ? with the increases of the dopant HCl concentration. Addition of polyaniline nanofibers layer with a 0,6 M HCl concentration shows a large shift in the SPR curve than with the addition of polyaniline nanofiber layer using other concentrations. This result due to the influences of n and ?value on polyaniline nanofibers. Thus, it can be concluded that the 0,6 M concentration of HCl was optimum for used to immobilize biomolecules on the surface sensing of SPR biosensors.

Kata Kunci : polyaniline nanofibers, interfacial polymerization, Kramers Kronig relationship, refractive index, extinction coefficient, SPR biosensors