VALIDASI METODE SPEKTROFOTOMETRI UNTUK ANALISIS SURFAKTAN ANIONIK DODESIL BENZENA SULFONAT (DBS) DALAM IKAN LELE (Clarias batracus) MENGGUNAKAN MALASIT HIJAU; VALIDATION OF SPECTROFOTOMETRIC METHOD FOR ANALYSIS OF ANIONIC SURFACTANT DODECYL BENZENE SULFONATE (DBS) IN CATFISH (Clarias batracus) USING MALACHITE GREEN

Hermawan Purba, Adhitasari Suratman

2014 | Disertasi | PROGAM STUDI S2 ILMU KIMIA

Validation method of DBS anionic surfactant analysis on Clarias batracus has been conducted. The method of analysis was divided into two phase, namely the extraction with solid-liquid extraction using Soxhlet and analysis DBS. The extraction was performed using n-hexane and methanol for 9 and 6 hours respectively. The analysis was performed using Spektrophotometer UV-Vis based on the complex formation of surfactant-malachite green (DBS-MG). These methods are applied to determine DBS accumulation of Clarias batracus with DBS concentration exposure and DBS concentration of Clarias batracus in markets. The result showed that the parameters of validation methods have high acceptability as linearity (R2 = 0.99), limit of detection (LOD) and limit of quantification (LOQ) (0.029 mg/L and 0.089 mg/L), sensitivity (? = 38.15 × 104 L mol-1 cm-1), precision (RSD = 0.10-1.83 %) and accuracy (recovery = 80-92 %). The result of analysis of DBS in Clarias batracus with 2.5; 5; 10; 15 mg/L of DBS concentration exposure obtained 5.5; 6.8; 7.9; 8.7 mg/L respectively and Clarias batracus from markets in a range 2.0-4.2 mg/L. The result showed that the analysis of DBS anionic surfactants using MG can be applied for Clarias batracus.

Kata Kunci : Validation methode, extraction, Clarias batracus, dodecyl benzene sulfonate, malachite green.