ENKAPSULASI KURKUMIN DALAM NANOPARTIKEL KITOSANPEKTIN TERTAUT SILANG GLUTARALDEHIDA DAN STUDI PELEPASANNYA SECARA IN VITRO; ENCAPSULATION OF CURCUMIN INTO GLUTARALDEHYDECROSSLINKED CHITOSAN-PECTIN NANOPARTICLES AND ITS IN VITRO DRUG RELEASE STUDY

GRASIANTO, Dwi Siswanta

2014 | Disertasi | PROGRAM STUDI S-2 KIMIA

An investigation of encapsulation of curcumin into glutaraldehydecrosslinked chitosan-pectin nanoparticles and its in-vitro drug release study had been conducted in order to determine the effectiveness of the nanoparticles to release curcumin in the releasing media. The composition of the components that influenced the curcumin release including chitosan, pectin and glutaraldehyde was studied. Nanoparticles were prepared using coacervation method by mixing chitosan and pectin, each was disssolved in 1% acetic acid solution, then added glutaraldehyde as crosslinker to the mixed solution. The prepared nanoparticles were characterized by FTIR, TEM, SEM, and XRD. In vitro drug release study was conducted by dispersing nanoparticles in the PBS:etanol (60:40) for 6 hours. The concentration of curcumin was determined spectrophotometrically. The kinetic of the curcumin release was analyzed by the several models; zero order model, first order model, Higuchi model and Korsmeyer-Peppas model. The shift of the chitosan-pectin’s characteristic peaks characaterized by FTIR indicated that there were interactions of functional groups. Size analysis studies revealed that nanoparticles have spherical morphology with mean diameter of about 30-40 nm. XRD results confirmed that curcumin in nanoparticle system has amorphous state. The results of this work showed that the optimum composition of nanoparticles was chitosan, pectin and glutaraldehyde respectively 15.59; 6.68; 75.50% which gave the optimum curcumin loading of 5.57 mg/g nanoparticles. The kinetic study revealed that nanoparticles tend to follow Korsmeyer-Peppas model equation through the release mechanism of fickian diffusion and polimer degradation with the constant rate of 3.345 menit-0,503. The optimum curcumin release of the nanoparticles reached 70.63% for 6 hours

Kata Kunci : nanopartikel; kurkumin; kitosan; pektin