PENGARUH BREED SAPI POTONG TERHADAP KUALITAS MORFOLOGI OOSIT YANG DIKOLEKSI SECARA IN VITRO

SEPTYABAYU, Agung Budiyanto

2014 | Skripsi |

Penelitian ini bertujuan untuk mengetahui pengaruh breed terhadap kualitas dari morfologi oosit yang telah dikoleksi secara in vitro, serta menentukan salah satu faktor yang menunjang efisiensi metode koleksi oosit untuk in vitro fertilization (IVF). Oosit diambil dari ovarium sapi potong dari Rumah Potong Hewan (RPH) Giwangan, Yogyakarta. Ovarium dibedakan berdasarkan breednya meliputi simPO, limPO, dan PO. Ovarium dibersihkan dari mesovarium dan lemaknya lalu dicuci menggunakan larutan 0,9 % NaCL fisiologis yang telah ditambah antibiotik Penicyllin-Streptomycin sebanyak dua kali pencucian lalu dimasukan ke dalam termos yang berisi larutan yang sama pada suhu 35º - 37º C untuk langsung dibawa ke laboratorium. Koleksi oosit dilakukan dengan aspirasi menggunakan spuit 10 ml dengan jarum 18 gauge yang telah diisi larutan Phospat Buffer Saline (PBS) sebanyak 0,5-1 ml. Cairan yang didapat tersebut dimasukan ke dalam tabung dan ditambah PBS 5ml, diendapkan 15 menit. Supernatan lalu dibuang dan sisanya dituang ke dalam cawan petri untuk diamati menggunakan mikroskop untuk diklasifikasi berdasarkan morfologinya. Hasil penelitian diolah menggunakan metode Anova (P<0,05). Hasil pengolahan data didapatkan kualitas oosit A, B, C, dan D adalah (P=0,60), (P=0,71), (P=0,314), dan (P=0,079) hasil menunjukan tidak ada perbedaan yang signifikan. Kesimpulan dari penelitian ini adalah breed pada sapi tidak berpengaruh terhadap kualitas oosit yang dikoleksi secara in vitro.

The main objective of conducting this research is to better understand the impact of breed towards the quality of oocyte morphology that has been collected through in vitro, and also determine one of factors that support the efficiency of oocyte collection method for in vitro fertillization.(IVF) The oocyte was taken from the ovarium of cattle which taken from abbatoir (RPH) in Giwangan, Yogyakarta. The ovarium is classified based on the breed which include simPO, limPO, and PO. It was cleaned from mesovarium and its fat, then it was being washed using 0.9% of physiological NaCL which has been added by Penicyllin-Streptomycin antibiotics for twice and then it put into a flask containing the same solution at a temperature of 35 º - 37 º C to be taken straight to laboratorium. Oocyte collection is done by aspiration using a 10 ml syringe with a 18 gauge needle that has been filled in Phosphate Buffer Saline solution (PBS) of 0.5-1 ml. The liquid obtained was put in a tube and added by 5ml of PBS, then precipitated for 15 minutes. The supernatant was then discarded and the remainder was poured into a petri dish to be observed using a microscope to be classified based on its morphology. The obtained results were anlyzed by Anova method (P<0,05). The result of the anlysis of the quality of oocytes obtained A, B, C, and D respectively (P=0,60), (P=0,71), (P=0,314), and (P=0,079) showed no significant difference. The conclusion of this study is that the breed does not affect the quality of the morphology of oocytes collected in vitro.

Kata Kunci : cow, breed, ovalium folicle, oocyte collection, oocyte quality, IVF