PENGARUH PEMBERIAN TAMOXIFEN PADA HATI TIKUS SPRAGUE DAWLEY PASCA OVARIEKTOMI

RUSTIASIH, Nur Wahyu , Devita Anggraeni

2014 | Skripsi |

Penelitian ini bertujuan untuk mengetahui pengaruh pemberian tamoxifen terhadap berat badan, rasio berat hati terhadap berat badan, gambaran makroskopik dan histopatologik hati tikus Sprague Dawley 2 bulan pasca ovariektomi. Sebanyak 8 ekor tikus (Sprague Dawley) betina dibagi dalam 2 kelompok, kelompok A tikus ovariektomi tanpa pemberian tamoxifen dan kelompok B tikus ovariektomi yang diberi tamoxifen 10 mg/kg berat badan. Masing-masing kelompok terdiri dari 4 ekor tikus. Pemberian tamoxifen dilakukan secara peroral selama 2 bulan. Tikus kemudian dinekropsi untuk pengambilan hati. Sebelum nekropsi, dilakukan penimbangan berat badan tikus untuk mengetahui berat badan akhir. Hati diambil dan ditimbang terlebih dahulu, kemudian dimasukan dalam larutan formalin 10% untuk pembuatan preparat histopatologik dengan pengecatan HE. Hasil analisis berat badan tikus ovariektomi tanpa pemberian tamoxifen (203,5 ±12,1gram) berbeda bermakna dibandingkan tikus ovariektomi yang diberi tamoxifen (182,5±6,5 gram) (P<0,05). Rasio berat hati terhadap berat badan tikus ovariektomi dengan pemberian tamoxifen (0,035±0,0021 gram) lebih besar dibandingkan tikus ovariektomi tidak diberi tamoxifen (0,032±0,00096gram) (P<0,05). Gambaran makroskopik hati pada tikus ovariektomi dengan pemberian tamoxifen tidak mengalami perubahan, namun gambaran histopatologik memperlihatkan perubahan berupa degenerasi melemak dan nekrosis. Berdasarkan hasil pengamatan dapat disimpulkan bahwa pemberian tamoxifen dengan dosis 10 mg/kg BB dapat mempengaruhi berat badan, rasio berat hati terhadap berat badan dan gambaran histopatologik hati pasca ovariektomi, tetapi tidak mempengaruhi gambaran makroskopik hati.

This research was aimed to study the effect of tamoxifen on body weight, liver weight to body weight ratio, macroscopic and histopathological figure of Sprague Dawley rat liver two months after ovariectomy. A total of 8 female rats (Sprague Dawley) were divided into 2 group of 4, group A was ovariectomized rats without administration of tamoxifen and group B was ovariectomized rat with administration of tamoxifen 10 mg / kg body weight. Tamoxifen was given orally for 2 months. Rats were then necropsied to take the liver. Liver were taken and weighed first, then put it in 10 % formalin solution for histopathological examination using Hematoxyline Eosin staining. The results showed that the body weight of ovariectomized rat without tamoxifen administration (203.5±12.1g) was significantly higher than ovariectomized rats with tamoxifen administration (182.5±6.5g) (P < 0.05). Ratio of liver weight to body weight of ovariectomized rats with tamoxifen administration (0,035 ± 0,0021g) was significantly higher than ovariectomized rats without tamoxifen administration (0,032±0,00096g) (P<0.05). Macroscopic figure of the liver of ovariectomized rats with tamoxifen administration was not shown any changes, however, there was fatty degeneration and necrosis histopatologically. Based on the results, it can be concluded that the administration of tamoxifen (10 mg / kg body weight) might affect body weight, liver weight to body weight ratio and histopathologic figure of liver 2 months after ovariectomy, however macroscopic figure of the liver was not affected.

Kata Kunci : ovariektomi, hati, tamoxifen