STUDI IN-VITRO AKTIFITAS ANTIBAKTERIAL Scurrula atropurpurea TERHADAP METHICILLIN-RESISTANT Staphylococcus aureus (MRSA)

SANDI, Novra Arya , Siti Isrina Oktavia Salasia

2014 | Skripsi |

Staphylococcus aureus diketahui merupakan bakteri penyebab utama nosocomial dan community-acquired infection pada manusia, penyebab mastitis klinis, subklinis dan kronis pada ternak perah. Meningkatnya insiden infeksi oleh S. aureus, khususnya methicillin-resistant Staphylococcus aureus (MRSA) membutuhkan strategi baru untuk mencegah kejadian infeksi dalam skala yang lebih luas yang didukung dengan adanya fenomena resistensi antibiotik. Penelitian ini bertujuan mengetahui potensi ekstrak benalu teh (Scurrula atropupurea) sebagai antibakteri Methicillin-Resistant Staphylococcus aureus (MRSA) secara in-vitro. Uji in-vitro dilakukan dengan metode difusi agar menggunakan media agar Müller-Hinton (MH) yang telah diinokulasi S. aureus, kemudian diberi paper disc metisilin 5 μg dan eritromisin 15 μg serta blank paper disc yang diberi ekstrak benalu teh konsentrasi 5%, 10%, 20% dan 40%. Hasil uji menunjukkan S. aureus bersifat resisten terhadap metisilin dan eritromisin dan ekstrak benalu teh mampu menghambat pertumbuhan S. aureus dengan zona hambatan melebihi zona standar herbal antibakterial (≥ 5,62 mm) pada semua konsentrasi ekstrak yang diujikan. Berdasarkan hasil penelitian dapat disimpulkan bahwa ekstrak benalu teh mampu menghambat pertumbuhan Methicillin-Resistant S. aureus.

Staphylococcus aureus is a bacteria known as a major cause of nosocomial and community-acquired infection in humans as well as clinical, subclinical and chronic mastitis in dairy cattles. The increasing incidence of infection by S. aureus, particularly methicillin-resistant Staphylococcus aureus (MRSA) requires new strategies to prevent the incidence of infection in a broader scale that compounded with the phenomenon of antibiotic resistance. This study aimed to determine the potential of parasite tea extract (Scurrula atropupurea) as antibacterial methicillin-resistant Staphylococcus aureus (MRSA) using in-vitro test. In-vitro test was performed by the agar diffusion method using Muller- Hinton (MH) agar that had been inoculated with S. aureus. Paper disc contained of methicillin and erythromycin 5 μg and 15 μg were used as control discs of antibiotic. Blank paper discs that contained of parasite tea extract with the concentration of 5%, 10%, 20% and 40% were used as an antibacterial substance. The test result showed that S. aureus was resistant to methicillin and erythromycin. Parasite tea extract with the concentration of 5%, 10%, 20% and 40% could inhibit the growth of S. aureus with inhibition zone exceeds the standard of antibacterial zone for herb (≥ 5.62 mm). Based on the results of this study could be concluded that the parasite tea extract could inhibit the growth of methicillin-resistant S. aureus.

Kata Kunci : Scurrula atropupurea, Methicillin-Resistant Staphylococcus, resistensi bakteri, antibakterial.