PENGARUH BODY CONDITION SCORE (BCS) SAPI POTONG TERHADAP KUALITAS MORFOLOGI OOSIT YANG DIKOLEKSI SECARA IN VITRO

RIDHO, Muh Mahfud Arrasyid , Agung Budiyanto

2014 | Skripsi |

Produksi embrio secara in vitro membutuhkan oosit yang berkualitas baik. Faktor yang dapat mempengaruhi kualias oosit adalah tingkat body condition score (BCS) dan metode koleksi. Tujuan penelitian ini adalah untuk mengetahui pengaruh BCS sapi potong terhadap kualitas morfologi oosit yang dikoleksi secara in vitro. Oosit diambil dari ovarium sapi potong di RPH Giwangan, Yogyakarta. Ovarium dikelompokkan berdasarkan BCS sapi antara 2 – <3 dan 3 – <4 pada skala 1 – 5. Ovarium dibersihkan dari lemak dan mesovariumnya kemudian dicuci dua kali dengan larutan 0,9 % NaCl fisiologis yang ditambah antibiotik Penicyllin-Streptomycin dan dimasukkan termos yang berisi larutan yang sama pada suhu 350-370 C kemudian dibawa ke Laboratorium. Koleksi oosit dengan aspirasi menggunakan spuit 10 ml yang berisi larutan Phospat Buffer Saline (PBS) 0,5 – 1 ml dengan jarum 18 g. Cairan folikel dimasukkan tabung dan ditambah PBS 5 ml, diendapkan 15 menit. Supernatan dibuang, sisanya dituang dalam cawan petri dan dilihat di bawah mikroskop untuk diklasifiksikan berdasarkan morfologinya (A, B, C, D). Data yang diperoleh diolah dengan T-test. Hasil analisis didapatkan kualitas oosit A, B, C, D dan A+B berturut-turut adalah (P=0,804), (P=0,596), (P=0,272), (P=1,000), (P=0,838) hasil menunjukkan tidak ada perbedaan yang signifikan. Kesimpulan dari penelitian ini bahwa BCS tidak berpengaruh terhadap kualitas morfologi oosit yang dikoleksi secara in vitro (P>0,05).

The production of embrio in vitro needs good quality oocytes. Factor that affected the quality of oocytes are Body Condition Score (BCS) and collecting method. The purpose of this study was to determined the effect of BCS to quality of oocytes morphology collected in vitro. Oocytes were collected from slaughter beef’s ovary at Giwangan slaughter house, Yogyakarta. The ovaries differentiated by the level of BCS between 2 - <3 and 3 - <4 on a scale of 1-5. Ovary cleaned and the mesovarium washed twice with 0.9 % NaCl physiological solution which has been coupled with Penicyllin - Streptomycin antibiotics and put into a flask containing with same solution at a temperature of 350-370 C and then brought to the laboratory. Oocyte collected by aspiration using a 10 ml syringe filled with a solution of Phosphate Buffer Saline (PBS) as much as 0.5-1 ml with 18 gauge needle. Follicular fluid were obtained inserted tube and added 5 ml PBS, precipitated 15 minutes. The supernatant was discarded and the remainder was poured in a petri dish and then viewed under a microscope to clasified based on morphologic (A, B, C, D). The obtained results were analyzed by T-test. The results of the analysis of the quality of oocytes obtained A, B, C, D and A + B respectively (P = 0.804), (P = 0.596), (P = 0.272), (P = 1.000), (P = 0.838) showed no significant difference. The conclusion of this study is that the BCS does not affect the quality of the morphology of oocytes collected in vitro (P> 0.05).

Kata Kunci : Sapi, Body Condition Score (BCS), folikel ovarium, koleksi oosit, kualitas oosit.