PENGARUH TAMOXIFEN TERHADAP JANTUNG TIKUS DUA BULAN PASCA OVARIEKTOMI

POERY, Ade Bondan Prasasta , Devita Anggraeni

2014 | Skripsi |

Penggunaan tamoxifen sebagai terapi pengganti estrogen menunjukkan efek beragam pada beberapa organ. Penelitian ini dilakukan untuk mengetahui pengaruh pemberian tamoxifen terhadap berat jantung, gambaran makroskopik dan histopatologik jantung tikus Sprague-Dawley dua bulan setelah ovariektomi. Tikus betina berumur ±2 bulan sejumlah 8 ekor dibagi menjadi 2 kelompok (kelompok A dan kelompok B), masing-masing 4 ekor, kelompok A yaitu tikus ovariektomi tanpa pemberian tamoxifen (diberi aquabides 1 ml/hari) dan kelompok B yaitu tikus ovariektomi dengan pemberian tamoxifen (10 mg/kgBB/hari). Seluruh tikus diberi pakan standar serta air minum secara ad libitum. Pemberian obat dilakukan selama 2 bulan setelah ovariektomi. Hasil penelitian menunjukkan tidak adanya perbedaan yang signifikan (P>0,05) antara berat jantung/100gBB tikus ovariektomi tanpa pemberian tamoxifen (0,4175±0,059 g) dan tikus ovariektomi yang diberi tamoxifen (0,4775±0,084 g). Secara makroskopik, jantung tikus ovariektomi tanpa pemberian tamoxifen dan tikus ovariektomi dengan pemberian tamoxifen tampak normal. Secara histopatologi, otot jantung tampak normal, serabut otot jantung bercabang, jaringan ikat terlihat jelas, nukleus sel otot jantung tampak normal berada di tengah dan dikelilingi sitoplasma berwarna pucat. Hasil pemeriksaan histopatologi aorta juga menunjukkan struktur yang normal pada tunika intima, tunika media, dan tunika adventisia, dinding endotel tidak menunjukkan kerusakan, sobek, akumulasi LDL, dan penyumbatan. Dengan demikian, pemberian tamoxifen selama 2 bulan pasca ovariektomi tidak berpengaruh terhadap berat jantung serta gambaran makroskopik dan histopatologik jantung dan aorta.

The use of tamoxifen as estrogen replacement therapy showed diverse effects on several organs. Therefore, this research was conducted to study the effect of tamoxifen on heart weight, macroscopic and histopathological figure of the ovariectomized rat heart. Eight of female rats at ±2 months of age were divided into 2 groups of 4 (group A and group B). Group A was ovariectomized without giving tamoxifen and group B was ovariectomized with administration of tamoxifen (10 mg/kg/day). Rats were fed standard diet and water was given ad libitum. Treatment was done for 2 months after ovariectomy. The results showed that there was no significant difference (P>0.05) between the heart weight/100g body weight of ovariectomized rat without tamoxifen (0.42±0.06g) and with tamoxifen (0.48±0.08g). Macroscopically, heart of ovariectomized rat without and with tamoxifenwere looked normal. Histopathologically, cardiac muscle was appeared normal, branched fibers of cardiac muscle, connective tissue was clearly visible, the nucleus of cardiac muscle cells was located in the middle and surrounded by pale cytoplasm. Aorta was also showed a normal structure in the tunica intima, tunica media, and tunica adventitia, meanwhile, endothelial wall was showed no damage, rips, LDL accumulation, and blockages. In conclusion, administration of tamoxifen for 2 months after ovariectomy had no effect on heart weight and macroscopic and histopathological figure of the heart and aorta.

Kata Kunci : Tamoxifen, ovariektomi, jantung