PROFIL SGOT DAN SGPT PADA SAPI POTONG YANG TERDIAGNOSA LEPTOSPIROSIS DI KECAMATAN SEYEGAN, MOYUDAN, DAN MINGGIR KABUPATEN SLEMAN

NOVITASARI, Danik , Guntari Titik Mulyani

2014 | Skripsi |

Leptospirosis adalah penyakit yang tersebar luas di seluruh dunia disebabkan oleh bakteri dari genus leptospira yang patogen dan bersifat zoonosis. Leptospirosis dapat menyerang hewan ternak, hewan liar, hewan piaraan, dan manusia. Leptospira menyerang organ seperti ginjal, hati, paru-paru, dan organ reproduksi. Penelitian ini bertujuan untuk mengetahui profil SGOT dan SGPT pada sapi potong yang terdiagnosa leptospirosis di Kecamatan Seyegan, Moyudan, dan Minggir Kabupaten Sleman. Sebanyak 147 ekor sapi diperiksa secara klinis dan diambil darahnya sebanyak 10 ml melalui vena jugularis dengan menggunakan venoject. Serum dipisahkan, kemudian dilakukan uji terhadap leptospira menggunakan Microscopic Agglutination Test (MAT) yang dilakukan di Balai Besar Penelitian Veteriner (Bbalitvet) Bogor. Uji ini dikatakan positif apabila terjadi aglutinasi antara antibodi dalam serum dan antigen serovar leptospira sebesar 50% pada pengenceran 1:100. Sampel yang positif MAT, dilakukan pengukuran kadar Serum Glutamic Oxaloacetic Transaminase (SGOT) dan Serum Glutamic Pyruvic Transaminase (SGPT). Apabila dijumpai peningkatan kadar SGPT dan atau SGOT diatas normal maka dilakukan pengukuran dalam jumlah sampel yang sama untuk digunakan sebagai pembanding. Kegiatan ini dilakukan di laboratorium Pramita, Yogyakarta. Hasil yang diperoleh dianalisis dengan menggunakan T-Test dan secara deskriptif. Hasil uji MAT menunjukkan bahwa 27 dari 147 sampel (18,37%) positif. Hasil pengukuran SGPT menunjukkan 7 dari 26 sampel (27%) memiliki kadar diatas normal. Dengan menggunakan T-Test diperoleh informasi bahwa perbedaan kadar SGPT antara sapi dengan MAT negatif (sehat) dengan sapi dengan MAT positif (leptospirosis) sangat signifikan (P<0,05). Pengukuran kadar SGOT tidak menunjukkan adanya peningkatan. Dari penelitian ini dapat disimpulkan bahwa: (1) 18,37% sapi potong di Kecamatan Seyegan, Moyudan dan Minggir Kabupaten Sleman terdiagnosa leptospirosis. (2) Sebanyak 27% sapi potong yang terdiagnosa leptospirosis di Kecamatan Seyegan, Moyudan, Minggir Kabupaten Sleman secara signifikan mengalami peningkatan SGPT. (3) Sapi potong yang mengalami peningkatan kadar SGPT terinfeksi leptospira serovar hardjo dan ichterohaemorrhagiae.

Leptospirosis is a disease which widely spread in the world. Leptospirosis is caused by pathogenic bacteria of leptospira genus and it is a zoonotic. Leptospirosis infect farm animals, wild animals, domestic animals, and humans. Leptospira attack organs such as the kidneys, liver, lungs, and reproductive organs. The aims of this research was to determine the profile of SGOT and SGPT in bovine leptospirosis in Seyegan, Moyudan, and Minggir Subdistrict Sleman Regency. A hundred and fourty seven cattles were used as a sampel. Ten milliliters blood were collected from the jugular vein using venoject. Serum was separated, for Microscopic Agglutination Test (MAT) were conducted in Bbalitvet Bogor. The MAT result is positive if agglutination between antibodies and antigens occurs in 50% dilution of 1:100 or more. If MAT was positive, liver function test was done to determine the content of Serum Glutamic Oxaloacetic Transaminase (SGOT) and Serum Glutamic Pyruvic Transaminase (SGPT). If the levels of SGOT or SGPT was found above normal, the measurement in the same sampel size would be done to use as a comparation. This test was performed at Pramita Laboratory, Yogyakarta. The results were analyzed using T-Test and descriptively. The MAT results showed that 27 from 147 samples (18,37%) were positive. A total of 7 from 26 samples (27%) showed elevated levels of SGPT. TTest results showed that there were very significant difference of SGPT levels between samples from positif MAT group (leptospirosis) and negative MAT groups (healthy). The levels of SGOT showed no increase from the normal value. From the research can be concluded that: (1) 18,37% cattles in the subdistrict Seyegan, Moyudan, Minggir Sleman Regency were diagnosed with leptospirosis. (2) A total of 27% cattles that were infected by leptospira showed elevated levels of SGPT significantly. (3) The cattles that have elevated levels of SGPT were infected by leptospira hardjo and ichterohaemorrhagiae serovar.

Kata Kunci : leptospirosis, leptospira, sapi potong, SGPT, SGOT