RESPON FISIOLOGIS TERHADAP PROFIL RASIO NEUTROFIL/LIMFOSIT PADA SAPI PERANAKAN ONGOLE YANG DITRANSPORTASIKAN SELAMA EMPAT JAM

Wulan Sari, Retnoi, Pudji Astuti

2012 | Skripsi |

Transportasi hewan dalam rangka pemenuhan kebutuhan masyarakat seringkali menimbulkan efek stres pada hewan jika prosedur pengangkutan yang dilakukan tidak mengutamakan kesejahteraan hewan. Stres akibat transportasi dapat menyebabkan neutrofilia, limfopenia dan peningkatan rasio neutrofil/limfosit (N/L) > 1,0 pada sapi. Penelitian ini bertujuan untuk mengetahui respon fisiologis terhadap profil rasio N/L pada sapi Peranakan Ongole (PO) yang ditransportasikan selama empat jam dan efektifitas rasio N/L sebagai indikator stres. Penelitian ini dilakukan dengan menggunakan lima ekor sapi PO dewasa berjenis kelamin betina. Pengambilan darah I dilakukan sesaat sebelum transportasi pukul 16.00 WIB. Sampel darah diambil melalui vena jugularis. Kemudian sapi ditransportasikan dari Wonogiri pukul 19.50 WIB. Sesampainya di Cangkringan pukul 00.30 WIB dilakukan pengambilan darah II. Dua hari kemudian dilakukan pengambilan darah III pukul 20.35 WIB. Sampel darah yang didapat diujikan di Laboratorium Patologi Klinik FKH UGM untuk mengetahui gambaran darah dan nilai absolut neutrofil dan limfosit, kemudian dihitung efektifitas rasio N/L sebagai indikator stres. Hasil yang diperoleh menunjukkan rerata rasio N/L sesaat sebelum sapi ditransportasikan adalah 0,96±0,55, setelah tiba dilokasi 1,73±0,83 dan dua hari pasca transportasi 0,64±0,61. Berdasarkan hasil selama tiga tahap periode pengamatan dapat disimpulkan bahwa: (1) transportasi selama empat jam mengakibatkan peningkatan rasio neutrofil/limfosit (N/L) meskipun secara statistik tidak signifikan; (2) rasio N/L dapat dijadikan sebagai indikator stres.

Animal transportation in order to fulfill society’s needed to often done without giving any attention to animal welfare. As a consequence that problem, the animal will get stress. Stress that happen to animal as the effect of transported from one place to another might cause neutrophilia, lymphopenia, and the increasing of neutrophil/lymphocyte ratio (N/L) > 1,0 in cows. The aim of this research was to find the physiological response to the profile of N/L ratio in Ongole crossbred that transported during four hours and also the effectiveness of N/L ratio as the animal’s stress indicator. This research was done by using 5 adult Ongole crossbred cows. The first blood sampling was done at 4 pm, before the Ongole crossbred cows were transported. Blood-sample was taken through jugular vein. Those Ongole crossbred cows were transported at 7.50 pm from Belikrejo, Saradan, Baturetno, Wonogiri. After arriving in Kuwang, Cangkringan, Sleman, Yogyakarta at 12.30 am, the second blood sampling was taken as the continuous term. Two days afterwards, at 8.35 pm, the third or the last blood sampling was done. The blood sample was tested in Clinical Pathology Laboratory of FKH UGM to find blood profile and the absolute score of neutrophil and lymphocyte, then the effectiveness of N/L ratio as the animal’s stress indicator. The result showed the average of N/L ratio before the transportation was 0,96±0,55, after the transportation was 1,73±0,83 and two days after the transportation was 0,64±0,61. Based on those three periods, the conclusions are: (1) transportation during four hours can increase neutrophil/lymphocyte (N/L) ratio, although statistically insignificant; (2) N/L ratio can be used as stress indicator.

Kata Kunci : RESPON FISIOLOGIS TERHADAP PROFIL RASIO NEUTROFIL/LIMFOSIT PADA SAPI PERANAKAN ONGOLE