Pengaruh Kualitas Oosit Sapi dengan Metode Koleksi Aspirasi dan Puncture Terhadap Kemampuan Maturasi Oosit In Vitro

MARSELA, Megasari, Asmarani Kusumawati

2012 | Skripsi |

Telah dilakukan penelitian pada ovarium sapi Wagyu atau Japanese Black Cow yang dikoleksi dari rumah potong hewan di Saga Prefecture, Jepang. Tujuan penelitian ini adalah untuk mengetahui pengaruh kualitas oosit sapi dengan metode koleksi aspirasi dan puncture terhadap kemampuan maturasi oosit in vitro. Oosit dikoleksi dari folikel berukuran 2-6 mm. Tujuh puluh ovarium dibagi secara acak dalam dua kelompok untuk koleksi oosit. Kelompok oosit satu dikoleksi dengan metode aspirasi, kelompok dua dengan metode puncture. Kemudian oosit dinilai kualitasnya dan dilakukan pematangan oosit pada inkubator bersuhu 38,5 oC 5% CO2 selama 20-22 jam. Koleksi oosit dengan menggunakan metode aspirasi menghasilkan 155 oosit, sedangkan koleksi oosit dengan menggunakan metode puncture menghasilkan 341 oosit. Dari metode aspirasi dihasilkan oosit dengan kualitas A: 45,8%, B: 27,1%, C: 14,8%, D: 12,3% dan dari metode puncture dihasilkan oosit dengan kualitas A: 16,1%, B: 13,8%, C: 31,4%, D: 38,7% dan berbeda nyata. Pada maturasi oosit, oosit grade A memiliki tingkat keberhasilan sebanyak 14,1% dari metode aspirasi dan 12,7% dari metode puncture, sementara oosit grade B memiliki tingkat keberhasilan sebanyak 9,5% dari metode aspirasi dan 8,5% dari metode puncture dan tidak berbeda nyata. Pada penelitian ini, dapat disimpulkan bahwa koleksi oosit dengan metode aspirasi dan puncture memiliki pengaruh yang signifikan terhadap kualitas oosit yang dihasilkan, namun tidak berpengaruh nyata pada jumlah oosit yang matur. Kata kunci : Maturasi oosit, koleksi oosit, oosit, metode aspirasi, metode puncture.

The aim of this study is to measure the effect of oocyte quality on collecting method towards aspiration and puncture regarding the ability of in vitro oocyte maturation in cattle. This study used ovaries of Wagyu the Japanese Black Cow which was collected in slaughter house in Saga Prefecture, Japan. After oovaries collecting in slaughter house,oocyte collecting is done. Oocyte was collected from follicle sized 2-6 mm. Seventy ovaries was divided randomly in two group of oocyte collecting. The first group was treated by aspiration method and the second one was treated by puncture method. Then, oocyte quality was evaluated and oocyte maturation was done in incubator at 38,5oC for 20-22 hours. Oocyte collecting which was used aspiration method produced 115 oocytes and puncture method produced 341 oocytes. Thus the oocyte quality of aspiration method were grade A: 45,8%, B:27,1%, C: 14,8%, D: 12,3% and the puncture method were grade A: 16,1%, B: 13,8%, C: 31,4%, D: 38,7%, significantly different. The percentage of grade A oocyte could mature were on aspiration method: 14,1%, on puncture method: 12,7%. The percentage of grade B oocyte could mature were on aspiration method: 9,5%, on puncture method: 8,5% and there is no significant difference between each group. From this study, the conclusion was, oocyte collection by using method of aspiration and puncute has significant difference regarding oocyte usable quality but it has no significant difference regarding the number of maturated oocyte. Key words: Oocyte maturation, oocyte collection, oocyte, aspiration method, puncture method.

Kata Kunci : Maturasi oosit, koleksi oosit, oosit, metode aspirasi, metode puncture.