Isolasi dan uj isensitivitas Escherichiacoli pada susu sap imastitis subklinis terhadap penisilin dan amoksisillin di kecamatan Pakem
ADITYA, Dendy, Yanuartono
2012 | Skripsi |Mastitismerupakansalahsatupenyakitpadasapiperahyangmenimbulkanbanyakkerugianbagiparapeternak,terutamapenurunanproduksidankualitassusu.Terapimastitisbiasanyaditanganidenganantibiotik,namunkarenapenggunaanantibiotikyangtidaktepatseringmenimbulkanresistensibakteri.Adanyakemungkinanbakteriresistenterhadapantibiotik,makaperludilakukanpengujiansensitivitasterhadapbakteripadasusu.TujuanpenelitianiniadalahuntukmengisolasibakteriE.colipadasapiperahmastitissubklinisdanmengetahuisensitivitasnyaterhadapPenisillindanAmoksisillin. Penelitianinimenggunakan20sampelsususapi,yangakandiisolasiterhadapadanyabakteriE.colidenganmenggunakanmediaisolasistandardanmediagula-gula.E.coliyangtumbuhakandiujisensitivitasnyaterhadapantibiotikdenganmetodediskdiffusiontest. Dari20sampelsususapimastitissubklinisyangdigunakan,bakteriE.colidapatdiisolasidari11sampel(55%).Hasilujisensitivitasmenunjukkanbahwa10isolat(91%)bakteriE.colisensitifterhadapAmoksisillindanhanya1isolat(9%)E.coliyangsensitifterhadapPenisillin.BerdasarkanujisensitivitasantibiotikterhadapisolatEscherichiacolidalampenelitianini,makadianjurkanpengobatanuntukmastitissubklinispadasapiperahdiPakemmenggunakanantibiotikamoksisillin.K atakunci:Escherichiacoli,sensitivitasantibiotik,amoksisillin,penisillin.
Mastitisisadiseaseindairycowswhichcausedmanylossestothefarmers,especiallythedecreaseinmilkproductionandquality.Treatmentofmastitisisusuallytreatedwithantibiotics,butduetotheinappropriateuseofantibioticsoftencausebacterialresistance.Becauseofthepossibilityofbacteriaresistancetoantibiotics,itisneccesarytotestthesensitivityofthebacteriainmilk.ThepurposeofthisstudywastoisolatethebacteriumEscherichiacoliinsubclinicalmastitisofdairycowsandtodeterminetheirsensitivitytoPencillinandAmoxycillin.Thisstudyusing20sampleofcow’smilkwhichwillbeisolatedtothepresenceofbacteriaE.colibyusingstandardisolationmediaandadditionalmedia.E.coliwhogrewuptobetestedsensitivitytoantibioticsbythediskdiffusiontestmethods. Ofthe20samplesofcow'smilkisusedsubclinicalmastitis,thebacteriumE.colicanbeisolatedfrom11samples(55%).Sensitivitytestresultsshowedthat10isolates(91%)E.colibacteriaaresensitivetoAmoxycillinandonly1isolates(9%)E.coliaresensitivetoPenicillin. BasedonantibioticsensitivitytestingofisolatesofEscherichiacoliinthisstudy,itisrecommendedforthetreatmentofsubclinicalmastitisindairycowsinPakemamoksisillinantibiotics. Keywords:Escherichiacoli,antibioticsensitivity,amoxycillin,penicillin.
Kata Kunci : Escherichiacoli, sensitivitas antibiotik,amoksisillin,penisillin.
