Pengaruh Perubahan Asupan Pakan Terhadap Kadar Kolesterol Darah Kambing Peranakan Ettawa

CAHYO, Ardika Eka , Irkham Widiyono

2012 | Skripsi |

Penelitian ini bertujuan untuk mengkaji pengaruh asupan pakan terhadap kadar kolesterol darah pada kambing peranakan ettawa (PE). Penelitian ini dilakukan dengan menggunakan kambing Peranakan ettawa (PE) sebanyak tiga ekor yang dipelihara dalam kandang individu, berumur kira-kira 1 tahun, jenis kelamin jantan dan secara klinis sehat. Kambing diberi perlakuan pakan menjadi tiga periode yaitu Periode I (Full feeding) dengan pemberian pakan secara ad libitum dilajutkan dengan Periode II (Restriction) dengan pemberian pakan dibatasi 50% dari jumlah awal dan diikuti dengan Periode III (Refeeding) dengan pemberian pakan kembali ad libitum. Lama setiap periode adalah empat minggu. Pengambilan sampel darah vena (5 ml) dilakukan pada minggu terakhir setiap periode ( minggu ke 4, 8 dan 12). Serum dipisahkan dengan cara sentrifugasi dan disimpan pada temperatur -200C sampai pemeriksaan dilakukan. Kadar kolesterol diukur dengan metoda spektrofotometrik. Perubahan kadar kolesterol selama periode perlakuan dianalisis dengan ANOVA (Analysis of Variance) dan dilanjutkan dengan DMRT (Duncan Multiple Range Test). Hasil analisis statistik dapat dinyatakan signifikan bilamana P<0,05. Rerata kadar kolesterol pada Periode I (Full feeding) sebesar 73,00±26,06 mg/dL, pada Periode II (Restriction) sebesar 104,33±28,02 mg/dL dan pada Periode III (Refeeding) sebesar 66,00±10,15 mg/dL. Penurunan asupan pakan mengakibatkan adanya peningkatan kadar kolesterol meskipun secara analisis statistik tidak menunjukan perbedaan yang nyata (P>0,05). Berdasarkan hasil penelitian dapat disimpulkan bahwa penurunan asupan pakan dapat mengakibatkan peningkatan kadar kolesterol di dalam serum. Kata Kunci : asupan pakan, kambing Peranakan ettawa, kolesterol.

This study was aimed to evaluate the effect of feed intake on blood cholesterol level in Ettawa crossbreed goats. This study was done by using three clinically healthy adult male Ettawa crossbreed goats. The goats were fed ad libitum for the first four weeks (Period I, Full feeding). During the weeks 5-8 the animals were fed 50% of the amount Period I (Full feeding) and during the week 9-12 the animals were fed ad libitum again. Five milliliters of blood samples were taken on week 4 of each period. Serum was separated by centrifugation and stored at -20oC until the chemical analysis were conducted. Cholesterol levels were measured by the spectophotometric method. Change of cholesterol level, during the period were analyzed by ANOVA (Analysis of Variance). The serum cholesterol concentration increased slightly as the feed intake was restricted (from 73,00±26,06 mg/dL in Period I to 104,33±28,02 mg/dL in Period II) and decreased again to the level of 66,00±10,15 mg/dL at the end of Period III (Refeeding). It could indicated that feed intake might have influence on serum cholesterol level in Ettawa crossbreed goats. Keywords: feed intake, Ettawa crossbreed goats, cholesterol.

Kata Kunci : asupan pakan, kambing Peranakan ettawa, kolesterol.