Kemampuan Fagositosis Sel Neutrofil terhadap Staphylococcus aureus Isolat Asal Karkas, Bumble Foot dan Arthritis pada Ayam secara In Vitro
YUNUARTI, Eka , Siti Isrina Oktavia Salasia
2012 | Skripsi |Kasus yang sering ditemukan pada ayam yaitu penyakit bumble foot dan arthritis. Sel neutrofil merupakan salah satu sel darah putih yang berperan dalam sistem pertahanan tubuh non spesifik terhadap infeksi bakterial. Penelitian ini bertujuan untuk mengetahui kemampuan fagositosis sel neutrofil terhadap Staphylococcus aureus isolat ayam yang berasal dari karkas, kasus bumble foot dan arthritis. Dalam penelitian ini digunakan 10 isolat Staphylococcus aureus, yang terdiri dari dua isolat asal karkas ayam, tiga sampel asal bumble foot (radang telapak kaki), dan lima sampel arthritis (radang sendi). Isolat berasal dari desa Kemiri Purwobinangun Pakem, Sleman, Yogyakarta. Untuk uji fagositosis digunakan darah kelinci yang diambil dari vena auricularis, ditampung dalam tabung dengan ethylenediaminetetraacetic acid (EDTA). Pemisahan sel neutrofil dilakukan dengan cara sentrifugasi 3.000 rpm selama 5 menit. Uji fagositosis secara in vitro dilakukan dengan cara menginkubasi 100 μL larutan bakteri (108 sel/mL) dicampurkan dengan 100 μL larutan sel neutrofil, selama 2 jam di dalam waterbath. Untuk mengetahui kemampuan sel neutrofil dalam memfagosit bakteri digunakan pewarnaan safranin 1% dan pengamatan dengan mikroskop cahaya. Bakteri yang difagosit akan tampak berwarna merah di dalam sel neutrofil. Hasil penelitian menunjukkan bahwa jumlah rata-rata Staphylococcus aureus isolat asal arthritis yang difagosit oleh sel neutrofil lebih sedikit (12,6 bakteri/sel) dibanding isolat bumble foot (15,9 bakteri/sel) dan karkas ayam (17,8 bakteri/sel) (p>0,05). Staphylococcus aureus isolat asal arthritis kemungkinan lebih patogen dibandingkan dengan isolat bumble foot dan karkas, karena Staphylococcus aureus isolat asal arthritis mampu menghindari aktivitas fagositosis sel neutrofil. Kata kunci: Staphylococcus aureus, karkas, bumble foot, arthritis, fagositosis, sel neutrofil
Bumble foot and arthritis are diseases that often attack the chickens. Neutrophil cells is one of the leukocytes that plays a role as non specific defense system against bacterial infection. The study aimed to determine the ability of neutrophil cells to phagocyte Staphylococcus aureus isolated from carcass, bumble foot and arthritis of chickens. In this study were used 10 isolates of Staphylococcus aureus, consisted of two isolated from chicken carcasses, three samples from bumble foot, and five samples of arthritis. All samples were obtained from the village of Kemiri Purwobinangun Pakem, Sleman, Yogyakarta. To phagocytic assay was used rabbit blood which was collected from auricular vein, and kept in the tubes containing of ethylenediaminetetraacetic acid (EDTA). Neutrophil cells were separated by centrifugation at 3.000 rpm for 5 minutes. In vitro phagocytic assay was performed by incubating 100 μL solutions of bacteria (10 8 cells/mL) and 100 μL solutions of neutrophils cells for 2 hours in a water bath. The ability of neutrophil cells to phagocyte the bacteria, was used 1% safranin staining and observed under the light microscope. Phagocytized bacteria will appear in red on the neutrophil cells. The results of the study showed that the average number of Staphylococcus aureus isolated from arthritis were phagocytosed by neutrophil cells fewest (12,6 bacteria/cell) in compare to the bumble foot isolates (15,9 bacteria/cell) and carcass isolates (17,8 bacteria/cell) (p>0,05). Staphylococcus aureus isolated from arthritis was probably more pathogen due to the ability to avoid the activity of neutrophil phagocytic cells. Key words: Staphylococcus aureus, carcass, bumble foot, arthritis, phagocytose, neutrophil cell
Kata Kunci : Staphylococcus aureus, karkas, bumble foot, arthritis, fagositosis, sel neutrofil
