Epidemiologi Verocytotoxigenic Escherichia coli (VTEC) pada sapi perah di Propinsi Jawa Tengah dan Daerah Istimewa Yogyakarta

SUMIARTO, Bambang, Promotor Prof.Drh. Setyawan Budiharta, MPh.,PhD

2002 | Disertasi | S3 Ilmu Pertanian

It has frequently been proven that verocytotoxigenic Escherichia coli (VTEC) muy cause dangerous human internal disorders such as haemorrhagic colitis, huemolytic uremic syndrome and thrombotic thrombocytic purpura. Evidence in some countries also indicated that food-producing animals act as reservoir of those diseases. Among reservoir animals, dairy cattle constituted the most important sources of VTEC infection. Knowledge on the epidemiology of the VTEC infection in dairy cattle is, tlzrefore, extremely helpful in preventing the spread of the disease to human. The ultimate goal of the current research is to describe the epidemiological patterns of VTEC infection in dairy cattle in Central Java and Yogyakarta Special Territory. A total number of I509 dairy cattle wkre sampled by a four stage sampling procedure in I0 dairy cattle densely populated areas, combined with a cluster sampling ut farm level. A number of putative risk factors at animal and farm levels were obtained by face to face interview in the study farms. Infection status of animals sampled was determined by faecal sample-isolated E. coli tested by enzyme-linked immunosorbent assay (ELISA) procedure. Monoclonal antibody used in the assay was produced uguinst local isolates of E. coli from dairy cattle proven as having VTI and VT2 genes. Monoclonal antibody was produced against whole as well as sonicated bacteria. Results of the ELISA were compared with those of latex agglutination test towards OIj7 and H7 antigens. Model building of the VTEC infection were constructed using logistic and least square linear regression procedures to create. a predictive model of the VTEC infection und prevalence model at the animal and firnilevel, respectively. Of the two types of the antigen used in the assay, antigen of the sonrJicated bacteria yielded better quality of the antibody than that of the whole bacteria as indicated by the fact that its absorbance levels were higher and ascites liquid produced was more voluminous than those of the whole bacteria. The result of the Cohen's Kappa statistic analysis between ELISA procedure using the monoclonal antibody of the sonicated bacterial antigen and latex agglutination test indicated that the two tests showed a Kappa coeficient of 0.6. It means that the two tests provide a good level of agreement. VTEC prevalence in Central Java and Yogyakarta Provinces at the animal level was 27.4 %, meanwhile at the farm level was 53.5 %. With regard to geographical distribution and disease prevalence, there was a significant dzference between geographical distribution and VTEC prevulence @

Kata Kunci : Epidemiologi Veteriner,Infeksi VTEC,Sapi Perah DIY, VTEC, monoklonal antibody, infiction model