RESPONS HISTOMORFOLOGIS JARINGAN ADIPOSA DAN INTESTINUM TENUE PUYUH (Coturnix coturnix japonica) BETINA DENGAN SUPLEMENTASI PROBIOTIK MAROLISTM

ARINA HASANAH, Zuliyati Rohmah, S.Si., M.Si., Ph.D.

2017 | Skripsi | S1 BIOLOGI

Penelitian ini bertujuan untuk mengetahui pengaruh suplementasi Probiotik MarolisTM terhadap histomorfologis jaringan adiposa dan intestinum tenue pada puyuh betina. Sebanyak 12 ekor puyuh betina dewasa (usia 9 minggu) dibagi secara acak dalam empat kelompok perlakuan dan dipelihara selama 84 hari. Probiotik MarolisTM diberikan dengan cara dilarutkan dalam air minum dengan konsentrasi: 0 ml/ekor/hari (K); 0,1 ml/ekor/hari (P1); 0,2 ml/ekor/hari (P2); dan 0,3 ml/ekor/hari (P3). Penelitian menggunakan Completely Randomized Design (CRD) faktor tunggal, data diuji ANOVA dan dilanjutkan dengan uji beda rerata Tukey. Hasil penelitian menunjukkan bahwa Probiotik MarolisTM berpengaruh terhadap histomorfologis jaringan adiposa, tinggi vili dan kedalaman kripta duodenum, kedalaman kripta jejunum, lebar vili dan kedalaman kripta ileum puyuh betina (P<0,05). Luas sel adiposa berturut-turut adalah 389,2 mikrometer2; 258,1 mikrometer2; 253,8 mikrometer2; dan 245,4 mikrometer2 untuk K, P1, P2, dan P3. Tinggi vili duodenum P2 (110,5 mikrometer) dan P3 (110,1 mikrometer) berbeda nyata dibandingkan kelompok perlakuan lainnya. Tinggi vili jejunum pada P1 (93.88 mikrometer) berbeda nyata dibanding dengan K (66.87 mikrometer). Sedangkan, tinggi vili pada ileum tidak berbeda nyata, demikian pula untuk untuk lebar vili duodenum dan jejunum semua kelompok perlakuan (P>0,05). Vili ileum paling lebar terdapat di kelompok perlakuan P3 (20,2 mikrometer). Kedalaman kripta duodenum dan ileum berbeda nyata pada kelompok perlakuan P3 (22,2 mikrometer dan 20,9 mikrometer). Kedalaman kripta jejunum pada kelompok perlakuan P2 (18,8 mikrometer) dan P3 (19,2 mikrometer) berbeda nyata dari kelompok perlakuan lainnya. Penelitian ini menunjukkan bahwa suplementasi Probiotik MarolisTM 0,2 ml/ekor/hari dan 0,3 ml/ekor/hari menurunkan luas sel adiposa dan memperbaiki kondisi histomorfologis intestinum tenue.

This study was conducted to investigate the effects of MarolisTM probiotic supplementation on adipose tissue and intestinal histomorpholgy of female quails. Twelve of 9 weeks old female quails were alloted into four groups and reared for 84 days. Supplementation of MarolisTM probiotic were given orally at dose of 0 ml/bird/day (K); 0,1 ml/bird/day (P1); 0,2 ml/bird/day (P2); and 0,3 ml/bird/day (P3). The size of adipocytes, height, width of villus, and the depth of crypt in intestinum tenue were observed. Data were analyzed with OneWay ANOVA and continued with Tukey Test. The results showed that MarolisTM probiotic treatment have an affect on the adipocytes size, duodenum's villus' height and crypt's depth, crypt's depth in jejunum, as well as ileum's villus width and crypt depth (P<0,05). Adipocytes size were 389,2 mikrometer2; 258,1 mikrometer2; 253,8 mikrometer2; 245,4 mikrometer2 for K, P1, P2, and P3 repectively. P2 (110,5 mikrometer) and P3 (110,1 mikrometer) in the duodenum had significantly different villus heights than the other treatment groups. P1 (93.88 mikrometer) in the jejunum had significantly different villus heights than group K (66.87 mikrometer). The height of villus in the ileum were not significantly different from each other (P>0,05). The width of villus in duodenum and jejunum of all groups were not significantly different (P>0,05). The widest ileum villus was found in P3 (20,2mikrometer). The crypt's depth in duodenum and ileum of P3 were 22,2 mikrometer and 20,9 mikrometer respectively. These were significantly different from other groups. For jejunum's crypt depth, P2 (18,8 mikrometer) and P3 (19,2 mikrometer) were significantly higher than other groups. This study indicated that supplementation of 0,2 ml/bird/day (P2) and 0,3 ml/bird/day (P3) of MarolisTM probiotic showed the optimum result to lower the size of adipocytes and improve the histological structure of intestinum tenue.

Kata Kunci : Adipose, Histomorphology, Intestinum tenue, MarolisTM probiotic, Quails.