UJI SENSITIVITAS BAKTERI Aeromonas hydrophila dan Edwardsiella tarda TERHADAP DAUN SAMBUNG NYAWA (Gynura procumbens Lour. Merr.)

ANY SAFITRI, Prof. drh. Kurniasih, MV. Sc., Ph. D.

2017 | Skripsi | S1 KEDOKTERAN HEWAN

Daun sambung nyawa (Gynura procumbens Lour Merr.) telah diketahui memiliki banyak manfaat untuk pengobatan, salah satunya sebagai antibakteri. Penelitian ini bertujuan untuk mengetahui sensitivitas bakteri Aeromonas hydrophila dan Edwardsiella tarda terhadap daun sambung nyawa (Gynura procumbens Lour. Merr.). Uji sensitivitas dilakukan dengan metode Kirby-Bauer. Biak murni yang telah didapat, ditanam menggunakan cutton swab pada Mueller Hinton Agar (MHA). Disk gentamicin, enrofloxacin, dan perasan daun sambung nyawa konsentrasi 25%, 50%, dan 100% diletakkan pada plat MHA lalu diinkubasi pada suhu 30 oC selama 24 jam. Derajat sensitivitas diketahui melalui pengukuran zona terang di sekeliling masing-masing disk.Zona terang yang diperoleh setelah dilakukan uji sensitivitas bakteri Aeromonas hydrophila terhadap daun sambung nyawa masing-masing diameter hambatan pada konsentrasi 25% 7,5 mm, konsentrasi 50% 8,5 mm, konsentrasi 100% 10 mm, gentamicin 23 mm dan enrofloxacin 37 mm. Uji sensitivitas bakteri Edwardsiella tarda terhadap daun sambung nyawa masing-masing diameter hambatan pada konsentrasi 25% 9 mm, konsentrasi 50% 9 mm, konsentrasi 100% 10 mm, gentamicin 18 mm dan enrofloxacin 33 mm. Hasil uji sensitivitas bakteri Aeromonas hydrophila dan Edwardsiella tarda terhadap daun sambung nyawa adalah diperoleh diameter hambatan yang paling besar pada konsentrasi 100% yaitu 10 mm. Nilai sensitivitas antibakteri perasan daun sambung nyawa tidak lebih baik daripada kontrol positif gentamicin dan enrofloxacin.

The leaf of sambung nyawa (Gynura procumbens Lour. Merr.) had known to have many benefits for the medication, such as to antibacterial agent. The research was to determine the bacterial susceptibility test of Aeromonas hydrophila and Edwardsiella tarda to the sambung nyawa leaves (Gynura procumbens Lour. Merr.). The susceptibility test performed on Kirby-Baeur method. The pure culture was grown with cutton swab to Mueller Hinton Agar (MHA). Disk of gentamicin, enrofloxacin, and extract of the leaves with concentration 25%, 50%, and 100% were placed on MHA then were incubated at 30oC for 24 hours. The suscepbility was known by measured inhibition zone around of the disc. The inhibition zone after the bacterial susceptibility test of Aeromonas hydrophila to the sambung nyawa leaves (Gynura procumbens Lour. Merr.) for each inhibition diameter of concentration were 25% 7,5 mm, 50% 8,5 mm, 100% 10 mm, gentamicin 23 mm, and enrofloxacin 37 mm. The inhibition zone after the bacterial susceptibility test of Edwardsiella tarda to the sambung nyawa leaves (Gynura procumbens Lour. Merr.) for each inhibition diameter of concentration were 25% 9 mm, 50% 9 mm, 100% 10 mm, gentamicin 18 mm, and enrofloxacin 33 mm. The result of the bacterial susceptibility test of Aeromonas hydrophila and Edwardsiella tarda to the sambung nyawa leaves (Gynura procumbens Lour. Merr.) was gotten the best inhibition zone in concentration 100% was about 10 mm. This result was not better than positive control of gentamicin and enrofloxacin.

Kata Kunci : uji sensitivitas, Aeromonas hydrophila, Edwardsiella tarda, sambung nyawa