PENGARUH METODE EKSTRAKSI TERHADAP KANDUNGAN ASAM LEMAK Enteromorpha compressa DAN Ulva lactuca DARI KABUPATEN GUNUNGKIDUL

UMRO MEINA , Dr. R.A. Siti Ari Budhiyanti, S.T.P., M.P. dan Dr. Nurfitri Ekantari, S.Pi., M.P.

2017 | Skripsi | S1 TEKNOLOGI HASIL PERIKANAN

Penelitian ini dilakukan untuk mengetahui pengaruh metode ekstraksi terhadap total dan jenis asam lemak pada Enteromorpha compressa dan Ulva lactuca yang diperoleh dari Pantai Drini Gunungkidul. Penelitian ini menggunakan kondisi alga segar dan kering. Metode ekstraksi yang digunakan yaitu metode FMB (Folch Method with Buffer) dan metode LRC (Lepage and Roy). Tahapan penelitian meliputi pengujian berat kering, kadar air, total lipid, dan kandungan asam lemak. Hasil penelitian menunjukkan bahwa berat kering alga Enteromorpha compressa dan Ulva lactuca masing-masing sebesar 18,87 persen dan 32,31 persen. Kadar air dipengaruhi oleh faktor spesies dan kondisi alga, sedangkan total lipid dipengaruhi oleh kondisi alga. Persentase kadar air alga segar berkisar 61,53 persen hingga 72,75 persen, sedangkan alga kering berkisar 8,13 persen hingga 9,05 persen. Persentase total lipid alga segar berkisar 2,42 persen hingga 2,48 persen, sedangkan alga kering berkisar 1,36 persen hingga 1,55 persen. Metode ekstraksi FMB memperoleh total dan jenis asam lemak yang lebih banyak dibandingkan dengan metode ekstraksi LRC. Persentase total asam lemak pada metode FMB berkisar 84,00 persen hingga 94,19 persen, sedangkan metode LRC berkisar 10,88 persen hingga 85,67 persen. Jenis asam lemak yang terdeteksi pada penelitian ini yaitu asam miristat (C14:0), asam pentadekanoat (C15:0), asam palmitat (C16:0), asam palmitoleinat (C16:1, n-7), asam stearat (C18:0), asam oleat (C18:1, n-9), asam linoleat (C18:2, n-6), asam linolenat (C18:3, n-6) dan asam arakidat (C20:0). Persentase PUFA tertinggi sebesar 14,22 persen pada Entermorpha compressa kondisi sampel kering dengan metode FMB.

This study aimed to understand the effect of extraction method on total and fatty acid profile of Enteromorpha compressa and Ulva lactuca obtained from Drini Beach, Gunungkidul. The present study were used fresh algae and dried algae. This research used FMB (Folch Method with Buffer) and LRC (Lepage and Roy) as extraction methods. The study consisted of measuring the yield of dry algae, water content, total lipid, and types of fatty acid. The yield of dry algae in Enteromorpha compressa and Ulva lactuca were 18,87 percent and 32,31 percent. The water content was affected by the species and condition of algae, while the total lipid was affected by the condition of algae . Water content of fresh algae was 61,53 percent until 72,75 percent, whereas the dried algae was 8,13 percent until 9,05 percent. The total lipid for fresh algae was 2,42 percent until 2,48 percent, whereas the dried algae was 1,36 percent until 1,55 percent. FMB method got greater total and more variety fatty acid compared to LRC method. The percentage of fatty acid in FMB method ranged of 84,00 percent until 94,19 percent, while the LRC method ranged of 10,88 percent until 85,67 percent. The types of fatty acids detected from the study were myristic acid (C14:0), palmitic acid (C16:0), palmitoleic acid (C16:1, n-7), stearic acid (C18:0), oleic acid (C18:1, n-9), linoleic acid (C18:2, n-6), linolenic acid (C18:3, n-6), and arachidic acid (C20:0). The highest PUFA percentage 14,22% was attained from dried Enteromorpha compressa with FMB method.

Kata Kunci : Kata kunci: asam lemak, Entermorpha compressa, metode ekstraksi, PUFA, Ulva lactuca / Keywords: Entermorpha compressa, extraction method, fatty acid, PUFA, Ulva lactuca