DETECTION OF THE SEROTYPE OF Avibacterium paragallinarum FROM BROILER FIELD ISOLATE USING HEMAGGLUTINATION INHIBITION TEST

KAR YEE, LOW, Prof. Dr. drh. A.E.T.H. Wahyuni, M.Si

2017 | Skripsi | S1 KEDOKTERAN HEWAN

INTISARI PENENTUAN SEROTIPE Avibacterium paragallinarum ISOLAT LAPANG BROILER DENGAN UJI HAMBATAN HEMAGLUTINASI Low Kar Yee Infectious coryza (IC) adalah penyakit sistem pernafasan bagian atas unggas yang bersifat opportunis dan menular yang disebabkan oleh Avibacterium paragallinarum. Penyakit ini mobiditasnya tinggi namun mortalitasnya rendah. Kerugian utama terjadinya penurunan produksi telur pada ayam petelur dan peningkatan feed conversion rasio (FCR) pada ayam pedaging. Dikenal ada tiga serotipe Av. paragallinarum yaitu serotipe A, B dan C. Laporan mengenai serotipe Av. paragallinarum di Indonesia masih sedikit. Penelitian ini dilakukan untuk menentukan serotipe Av. paragallinarum dari ayam broiler uji hambatan hemaglutinasi (HH). Sebanyak tiga isolat lapang Av. paragallinarum diidentifikasi ulang kemudian dibuat antigen dengan menggunakan sonikasi untuk uji hemaglutinasi (HA). Titer 4HA unit telah diperoleh, kemudian diuji HH dengan menggunakan antisera referens (strain 221 serotipe A, strain Spross serotipe B, dan strain Modesto serotipe C). Hasil menunjukkan bahwa ketiga isolat tersebut menunjukkan titer tertinggi dengan antiserum serotipe B yaitu sebesar >5120 HH unit. Dapat disimpulkan bahwa ketiga isolat lapang Av. paragallinarum dari ayam pedaging mempunyai serotipe B.

Infectious coryza (IC) is an opportunistic and infectious upper respiratory tract disease in poultry, caused by Avibacterium paragallinarum. This disease has high morbidity but low mortality. The major losses caused by IC are the reduced egg production in layer chickens and increased feed conversion ratio (FCR) in broiler chickens. There are three recognized Av. paragallinarum serovars, which are serovar A, B and C. Limited reports regarding the serovars of Av. paragallinarum in Indonesia are available. This research was done to detect the serovar of Av. paragallinarum in broiler chickens using hemagglutination inhibition (HI) test. Three field isolates of Av. paragallinarum were re-identified, then processed into antigen solution using sonication, further on used for hemagglutination (HA) test. After the 4HA unit titer was obtained, the antigens were tested with HI using reference antisera (strain 221 serovar A, strain Spross serovar B, and strain Modesto, serovar C). The result showed that all isolates had the highest titer with antiserum serovar B which was >5120 HI unit. Therefore, it can be concluded that all three field isolates of Av. paragallinarum from broiler chickens are serovar B.

Kata Kunci : Avibacterium paragallinarum, serotipe, hambatan hemaglutinasi, ayam broiler