STUDI RESPON IMUN SELULER TERHADAP INFEKSI INTRAPERITONEAL ENTEROTOXIGENIC Staphylococcus aureus PADA MENCIT Balb/c

ADIBA, ABDUL RAHMAN, Prof. Dr. drh. Siti Isrina Oktavia Salasia

2017 | Skripsi | S1 KEDOKTERAN HEWAN

Respons imun seluler pada hospes dapat teraktivasi akibat dari infeksi bakteri. Dalam penelitian ini ingin diketahui respon imun seluler Staphylococcal enterotoxin B (SEB) pada mencit Balb/c. Dua kelompok mencit jantan Balb/c digunakan. Setiap kelompok terdiri dari enam mencit berumur lapan minggu, dan dilabel sebagai kelompok "Kontrol" dan "Uji". Infeksi SEB dilakukan secara intraperitoneal dengan volume 200 mL dengan konsentrasi suspensi 108 pada H-0 dan infeksi berikutnya dengan volume 600 mL dengan konsentrasi suspensi 108 pada H-13. Sampel darah dari enam mencit dikoleksi melalui pleksus retro orbitalis pada H-1, H-4, H-6, H-14, dan H-22. Leucocytes differential count dihitung secara manual dan dibandingkan dengan menggunakan perhitungan statistik. Peningkatan neutrofil muda dan limfosit nyata terlihat. Pada H-1, H-4, dan H-6 menunjukkan perbedaan yang signifikan dari jumlah leukosit terhitung dengan nilai H-1 2,67 x 103mm-3, H-4 4,12 x 103mm-3, dan H-6 5,08 x 103mm-3. Hasil penelitian menunjukkan adanya peningkatan pada produksi dan sirkulasi neutrofil muda (0,00 x 103 mm-3 pada H-0 ke 0.52 x 103 mm-3 pada H-22) , eosinofil (0,04 x 103 mm-3 pada H-1 ke 0,25 x 103 mm-3 pada H-22), monosit (0,18 x 103 mm-3 pada H-1 ke 0,37 x 103 mm-3 pada H-22), dan limfosit (1,80 x 103 mm-3 pada H-1 ke 3,77 x 103 mm-3 pada H-6) di pembuluh darah perifer setelah infeksi bakteri. Tiga mencit dieutanasi menggunakan kloroform dan dinekropsi pada H-7, dan tiga lagi pada H-22 dan perubahan histopatologi organ ginjal dan hati diamati. Pemeriksaan histopatologi menunjukkan infiltrasi leukosit di ginjal dan hati, serta erosi membrana mukosa epitel organ. Ada juga infiltrasi leukosit dan erosi pada membrana mukosa epitel organ.

Bacterial infections commonly will activate the cellular immune response of the infected hosts. Our laboratorical observations suggested that staphylococcal enterotoxin B (SEB) do have a prognostic differences in the cellular immune response. Two groups of male Balb/c mice were used. Each group consisted of six mice aged eight weeks, of which labelled as the "Control" group and the "Test" group. Infection of SEB were carried out intraperitoneally with the volume of 200 µL with the concentration of the suspension 108 on D-0 and a subsequent infection of SEB with the volume of 600 µL with the concentration of the suspension 108 on D-13. Blood samples were collected through the retro-orbital plexus on Day-1, D-4, D-6, D-14, and D-22. Leucocytes differential count were summed manually and compared using statistics. Leucocytes differentials were counted and the results between "Control" group and "Test" group were compared. Increased of band neutrophils (from 0.00 x 103 mm-3 in D-0 to 0.52 x 103 mm-3 in D-22), eosinophils (from 0.04 x 103 mm-3 in D-1 to 0.25 x 103 mm-3 in D-22), monocytes (from 0.18 x 103 mm-3 in D-1 to 0.37 x 103 mm-3 in D-22), and lymphocytes (from 1.80 x 103 mm-3 in D-1 to 3.77 x 103 mm-3 in D-6) were markedly seen. D-1, D-4, and D-7 showed significant differences of total leucocytes count of 2.67 x 103mm-3, 4.12 x 103mm-3, 5.08 x 103mm-3 respectively. Three mice were necropsied on D-7, and another three on D-22 to observed histopathological changes in the kidney and liver. Histopathological examination showed infiltration of leucocytes in kidney and liver, as well as erosion of the epithelial mucosal membrane of the organs. There are significant increased on the production and circulation of peripheral band neutrophils and lymphocytes following bacterial infection. There are also infiltration of leucocytes and erosion of the epithelial mucosal membranes of the organs.

Kata Kunci : Staphylococcus aureus, Enterotoxin B, Balb/c, intraperitoneal, hematologi, histopatologi