KEMAMPUAN AIR REBUSAN DAUN SALAM (Eugenia polyantha Wight) DALAM MENURUNKAN JUMLAH KOLONI BAKTERI PLAK

TIARA BUDI EKA LESTARI, Dr. drg. Juni Handajani, M. Kes, Ph.D ; drg. Tetiana Haniastuti, M. Kes, Ph.D

2017 | Skripsi | S1 HIGIENE GIGI

Plak adalah lapisan tipis yang melekat pada permukaan gigi dan struktur keras lain di rongga mulut. Plak terdiri dari 70% bakteri, seperti Streptococcus mutans, Actinomices naeslundii, Streptococcus sanguinis, Niesseria sp., dan lain-lain. Daun salam (Eugenia polyantha Wight) mengandung favonoid, tanin, dan minyak atsiri yang memilki aktifitas antibakteri. Penelitian ini bertujuan untuk mengetahui efek rebusan daun salam terhadap pertumbuhan bakteri plak. Sampel plak diambil dari permukaan bukal gigi molar pertama atas. Sampel kemudian diinokulasi pada media BHI dan diinkubasi dengan suhu 37o C selama 24 jam. Rebusan daun salam konsentrasi 50% (uji) dan akuades (kontrol) ditambahkan kedalam suspensi bakteri 1,5 x 108 cfu/ml. Tabung reaksi diinkubasi selama 24 jam pada suhu 37o C. Hasil inkubasi dimasukkan ke dalam BHI agar dan diinkubasi selama 24 jam pada suhu 37o C. Jumlah koloni dihitung secara visual. Analisis data menggunakan t-test. Hasil menunjukkan jumlah koloni pada kelompok uji lebih sedikit dari kelompok kontrol. T-test menunjukkan perbedaan tidak bermakna jumlah bakteri antar kelompok (p>0,05). Kesimpulan penelitian ini yaitu rebusan daun salam tidak efektif dalam menurunkan jumlah koloni bakteri plak.

Plaque is a thin layer attached to the tooth surface and other hard structures in the oral cavity. It consists of 70% bacteria such as Streptococcus mutans, Actinomyces naeslundii, Streptococcus sanguinis, Niesseria sp., etc. Bay leaf (Eugenia polyantha Wight) contains flavonoids, tannins, and essesial oils which has antibacterial activity. This study aimed to determine the effect of bay leaf decoction on growth of plaque bacteria. Plaque was taken from the buccal surface of upper first molar. The sampel was then inoculated in BHI media and incubated at 37oC for 24 hours. Fifty percent bay leaf decoction (treatment) and distilled water (control) was added into 1,5 x 108 CFU /ml bacterial suspension. The tube was incubated for 24 hours at 37oC. Sampel was cultured on BHI agar, then incubated at 37oC for 24 hours. The number of colonies grow on the agar were counted visually. Data were analyzed using T-test. Result showed the number of colonies of the treatment group was lower than the control group. T-test showed no significant difference between the groups (p>0.05). In conclusion, 50% bay leaf decoction is not effective in reducing the number of bacterial colonies plaque.

Kata Kunci : bakteri plak, rebusan daun salam, jumlah koloni