DISTRIBUSI KURKUMIN DAN KURKUMINOID PADA EMPU DAN ANAKAN RIMPANG Curcuma mangga Val. BERDASARKAN PERBEDAAN WAKTU PANEN

FEBRY DIFTA ROSALINA, Dr. Endang Astuti, M.Si; Sugeng Triono, S.Si, M.Si

2017 | Skripsi | S1 KIMIA

Telah dilakukan penelitian tentang distribusi kurkumin dan kurkuminoid yaitu demetoksikurkumin dan bisdemetoksikurkumin pada empu dan anakan rimpang Curcuma mangga Val. dengan waktu panen umur 3 dan 10 bulan. Penelitian ini bertujuan untuk mengetahui distribusi kukumin, demetoksikurkumin dan bisdemetoksikurkumin pada empu dan anakan rimpang Curcuma mangga Val. berdasarkan perbedaan waktu panen. Penelitian diawali dengan isolasi kurkumin dan kurkuminoid menggunakan metode ekstraksi maserasi dengan etanol 95% selama 24 jam. Kurkumin dan kurkuminoid diidentifikasi dengan KLT menggunakan eluen diklorometana:metanol (98:2 v/v) dan dimurnikan menggunakan metode kromatografi kolom. Hasil yang diperoleh ditentukan kadar senyawa kurkumin dan kurkuminoid menggunakan TLC Scanner dan dianalisis dengan LC-MS. Hasil penelitian menunjukkan bahwa distribusi kurkumin dan kurkuminoid lebih besar pada empu dibanding anakan rimpang Curcuma mangga Val. Tanaman Curcuma mangga Val. yang berumur 3 bulan memiliki kadar demetoksikurkumin>kurkumin>bisdemetoksikurkumin, dan tanaman yang berumur 10 bulan memiliki kadar kurkumin>demetoksikurkumin>bis-demetoksikurkumin. Berdasarkan hasil analisis dengan LC-MS, metode pemisahan dengan kromatografi kolom menggunakan eluen dikloro-metana:metanol (98:2 v/v) tidak dapat memisahkan ketiga senyawa tersebut.

The research about the distribution of curcumin and curcuminoid namely demethoxycurcumin and bisdemethoxycurcumin on parents and sapling rhizomes of Curcuma mangga Val. at 3 and 10 months harvest ages had been done. The purpose are determining the distribution of curcumin, demethoxycurcumin, and bisdemethoxycurcumin from parents and sapling rhizomes of Curcuma mangga Val. based on harvest time difference. The research was started with isolation of curcumin and curcuminoid using maceration extraction method with 95% ethanol for 24 h. Curcumin and curcuminoid been identified using TLC method with dichloromethane:methanol (98:2 v/v) eluent, and was separate using column chromatography. The compositon of curcumin and curcuminoid had determined by TLC Scanner and analyzed using LC-MS. The result of this research showed that the distributions of curcumin and curcuminoid were greater in parent than sapling rhizomes of Curcuma mangga Val. The level contens in 3 months Curcuma mangga Val. were demethoxy-curcumin>curcumin>bisdemethoxycurcumin, and the level contens in 10 months plant were curcumin>demethoxycurcumin>bisdemethoxycurcumin. According to LC-MS analysis, separation method by column chromatography using dichloro-methane:methanol (98:2 v/v) eluent was not separating all these compounds.

Kata Kunci : Curcuma mangga Val., kurkumin dan kurkuminoid, kromatografi kolom